芒果ANR基因的克隆及其表达分析

发布时间：2018-07-05 16:41

本文选题：芒果 + 原花青素　；参考：《江苏农业科学》2017年04期

【摘要】：花青素还原酶(anthocyanidin reductase,简称ANR)基因是植物产生原花青素的关键基因,对于研究原花青素的代谢有重要的作用。根据已经报道的ANR基因的序列设计兼并引物,采用3'c DNA末端快速扩增(3'RACE)、5'c DNA末端快速扩增(5'RACE)方法,从芒果果实内克隆到1个ANR基因,其全长c DNA序列为1 201 bp。该基因开放阅读框为1 008 bp,编码335个氨基酸,等电点为5.41,分子量为36.33 ku。对基因组扩增得到了1 810 bp长度的片段,分析发现,该基因含有5个内含子,内含子位置分别为130~646 bp、733~814 bp、1 017~1 080 bp、1 259~1 349 bp、1 563~1 651 bp。通过系统发育树分析发现,该基因编码的蛋白与可可、葡萄等果树具有较近的亲缘关系。对不同芒果品种中ANR基因的表达进行分析发现,该基因在绿色的桂七品种中表达量较高,而在红色的贵妃品种中表达量较低。
[Abstract]:Anthocyanin reductase (anthocyanidin reductase,) gene is the key gene for proanthocyanidins production in plants and plays an important role in the study of proanthocyanidins metabolism. According to the reported sequence of ANR gene, a degenerate primer was designed and cloned from mango fruit by rapid amplification (3race) and rapid amplification (5race). The full-length cDNA sequence of ANR gene was 1 201 BP. The open reading frame of the gene is 1 008 BP, encoding 335 amino acids, the isoelectric point is 5.41, and the molecular weight is 36.33 ku. The length of 1 810 BP fragment was obtained by genomic amplification. It was found that the gene contained 5 introns, the position of which was 130 ~ 646 BP ~ 733 ~ 814 BP ~ (1 017) ~ 1 080 BP ~ 1 259N ~ (1 349) BP ~ (1 349) BP ~ 1 563N ~ (1 651) BP respectively. Phylogenetic tree analysis showed that the protein encoded by the gene was closely related to the fruit trees such as cocoa and grape. The expression of ANR gene in different mango varieties was analyzed. It was found that the expression of ANR gene was higher in the green seven cultivars than in the red ones.
【作者单位】：荆楚理工学院生物工程学院;中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开发实验室;国家热带果树品种改良中心;
【基金】：国家自然科学基金(编号:31471850) 农业部热带作物种质资源保护(编号:15RZZY-07);农业部“948”计划(编号:2011-G13) 湖北省荆门市科技局项目(编号:110588)
【分类号】：S667.7

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