FASN基因的多态性及其与中国荷斯坦奶牛产奶性状的相关性分析

发布时间：2018-07-06 20:39

  本文选题：荷斯坦奶牛 + 脂肪酸合成酶(FASN)　； 参考：《黑龙江畜牧兽医》2017年11期

【摘要】：为了分析脂肪酸合成酶(fatty acid synthase,FASN)基因的多态性及其与中国荷斯坦奶牛产奶性状的相关性,试验选取230头荷斯坦奶牛,采用限制性片段长度多态性聚合酶链反应(Restriction fragment length polymorphism-polymerase chain reaction,RFLP-PCR),对FASN基因3'-UTR区和外显子部分片段的多态性进行检测,并对突变位点与奶牛产奶性状的相关性进行分析。结果表明:在所分析的片段中,检测到3'-UTR区存在一个C→T碱基的置换突变(g.51403203CT);该突变位点为Bam HⅠ酶的自然酶切位点,酶切后存在CC、TC、TT三种基因型,TT基因型的乳脂率和脂蛋白比显著高于TC基因型、CC基因型(P0.05),而三种基因型间乳蛋白率和305 d产奶量差异不显著(P0.05)。
[Abstract]:In order to analyze the polymorphism of fatty acid synthase (fatty acid synthase) gene and its association with milk production traits in Chinese Holstein cows, 230 Holstein cows were selected. Restriction fragment length polymorphism-polymerase chain polymorphism polymerase chain reaction (RFLP-PCR) was used to detect the polymorphism of FASN gene in 3r-UTR region and exon, and the correlation between mutation site and dairy cow milk yield traits was analyzed. The results showed that there was a C-T base replacement mutation (g. 51403203CT) in the 3H-UTR region, which was the natural enzyme digesting site of the Bam H 鈪，

本文编号：2104018