闽西地区新流行猪伪狂犬病病毒gD基因分子特征

发布时间：2018-07-07 09:19

本文选题：猪伪狂犬病病毒 + gD基因　；参考：《中国兽医学报》2017年05期

【摘要】：本研究收集闽西不同地区的17株猪伪狂犬病病毒(PRV)分离株,并对其gD基因进行遗传变异分析,探讨2013—2015年闽西地区PRV流行株的现状、分子生物学特征和遗传演化规律。结果显示,17株PRV分离株与7株PRV国内分离株核苷酸及氨基酸序列的同源性较高均为99.0%~100.0%;与4株国外PRV经典株的核苷酸及氨基酸序列同源性相对较低,分别为98.6%~99.3%,97.0%~99.0%。核苷酸多序列比对结果显示,17株PRV分离株最具有特征性的变化是在831~836bp插入了连续的6个碱基CCGGCC,进而导致gD氨基酸序列275~276位增加了2个氨基酸RP。抗原性分析结果显示,这2个氨基酸还位于gD蛋白的抗原表位区内。遗传进化树分析结果显示,17株PRV分离株与7株国内分离株、韩国Yangsan株及美国Becker株位于一个相对独立的遗传分支内,亲缘关系较近;与Bartha及Kaplan 2株匈牙利分离株位于不同的大遗传进化分支中,亲缘关系较远。
[Abstract]:In this study, 17 strains of porcine pseudorabies virus (PRV) isolated from different regions of western Fujian were collected and their GD gene genetic variation was analyzed. The present situation, molecular biological characteristics and genetic evolution of PRV epidemic strains in Western Fujian from 2013 to 2015 were discussed. The results showed that the homology of nucleotide and amino acid sequences between 17 PRV isolates and 7 domestic PRV isolates was 99.0100.00.The nucleotide and amino acid sequence homology was relatively low with four foreign PRV classical strains (98.6R 99.397.0) and 99.0% (P < 0.05), respectively. The results showed that the nucleotide sequence and amino acid sequence homology of 17 PRV isolates and 7 domestic PRV isolates were 99.0% and 99.0%, respectively. The results of nucleotide multiple sequence alignment showed that the most characteristic change of 17 strains was the insertion of 6 consecutive bases into the 831~836bp, which resulted in the addition of 2 amino acids to the amino acid sequence 275 ~ 276. The results of antigenicity analysis showed that the two amino acids were also located in the epitope region of GD protein. Phylogenetic tree analysis showed that 17 PRV isolates were closely related to 7 domestic isolates, Yangsan strain in Korea and Becker strain in the United States were located in a relatively independent genetic branch. The two Hungarian isolates, Bartha and Kaplan, were located in different branches of genetic evolution, and were closely related to each other.
【作者单位】：龙岩学院生命科学学院;福建省生猪疫病防控工程技术研究中心;
【基金】：国家自然科学基金青年科学基金资助项目(31602048) 福建省农业科技重大专项基金资助项目(2014NZ0002-3) 福建省科技厅自然科学基金资助项目(2016J01712) 龙岩学院产学研合作资助项目(LC2015010)
【分类号】：S852.651

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