腹腔注射亚精胺对小鼠卵巢组织多胺含量及代谢相关基因表达的影响

发布时间：2018-07-08 09:43

  本文选题：亚精胺 + 鼠　； 参考：《华南农业大学学报》2017年04期

【摘要】：【目的】研究腹腔注射亚精胺对鼠卵巢组织多胺代谢的影响。【方法】给鼠腹腔注射不同剂量亚精胺[0(对照组、0.05、0.10和0.15 mg·g~(-1)],应用实时荧光定量PCR检测多胺代谢关键基因表达量,应用高效液相色谱检测鼠卵巢组织中多胺含量。【结果】注射0.15 mg·g~(-1)亚精胺时,卵巢组织ODC、OAZ1、SPMS、SSAT、PAOX和SMOX基因表达量显著高于其他3组;注射亚精胺组鼠卵巢组织SPDS表达量均显著低于对照组;注射0.10 mg·g-1亚精胺组鼠卵巢组织中腐胺和亚精胺含量均显著高于对照组,而注射亚精胺对卵巢中精胺含量无显著影响。【结论】外源性亚精胺的腹腔注射可导致鼠卵巢组织中多胺含量以及多胺代谢相关基因表达量发生变化,且具有剂量依赖性,提示外源性亚精胺可通过介导卵巢组织的多胺代谢来参与调控卵巢功能。
[Abstract]:[objective] to study the effect of intraperitoneal injection of spermidine on the metabolism of polyamines in ovarian tissues of rats. [methods] different doses of spermidine were injected intraperitoneally into mice [0 (control group: 0.050.10 and 0.15 mg g ~ (-1)], and real-time quantitative PCR was used to detect the expression of key genes in polyamine metabolism. High performance liquid chromatography (HPLC) was used to detect the content of polyamines in mouse ovary. [results] the expression of ODCOAZ1 SPMSMSSSATPAOX and SMOX genes in ovarian tissue was significantly higher than that in the other three groups after 0.15 mg g ~ (-1) spermidine injection. The expression of SPDS in ovarian tissue of rats injected with spermidine was significantly lower than that of control group, and the contents of putrescine and spermidine in ovarian tissue of rats injected with 0.10 mg / g spermine were significantly higher than those in control group. There was no significant effect of spermidine injection on the content of spermine in ovary. [conclusion] Intraperitoneal injection of exogenous spermidine can result in changes of polyamine content and the expression of polyamine metabolism-related genes in ovarian tissue in a dose-dependent manner. The results suggest that exogenous spermidine can regulate ovarian function by mediating the metabolism of polyamines in ovarian tissues.
【作者单位】： 四川农业大学动物科技学院/畜禽遗传资源发掘与创新利用四川省重点实验室;
【基金】：国家自然科学基金(31201798) 高等学校博士学科点专项科研基金资助课题(20105103120003)
【分类号】：S814.1
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