梅花鹿鹿茸尖端组织Tβ-4基因全长cDNA的分离与生物信息学分析

发布时间：2018-07-08 10:23

本文选题：鹿茸 + 胸腺素β　；参考：《野生动物学报》2017年02期

【摘要】：胸腺素β4(Thymosinβ4,Tβ-4)是真核生物细胞中的一种具有多重生物学功能的肌动蛋白螯合分子。本研究首次从梅花鹿鹿茸尖端组织全长cDNA文库中成功克隆了Tβ-4基因的全长cDNA序列,并结合生物信息学方法对该基因的氨基酸序列进行了分析。结果表明,梅花鹿Tβ-4基因cDNA全长为770 bp,编码44个氨基酸,相对分子质量为5 052.65 u,理论等电点为5.02,其一级结构中赖氨酸和谷氨酸残基所占比例最高,二级结构元件主要以α-螺旋和无规则卷曲为主。结构分析发现,Tβ-4蛋白氨基酸序列含有"Thymosin family"结构域,没有N端信号肽,没有跨膜区。同源序列分析表明,梅花鹿与白颊长臂猿、人、牛的亲缘关系较近,氨基酸同源性高于85%。Tβ-4在鹿茸尖端组织全长cDNA文库中高丰度表达,提示它可能在鹿茸生长发育中起到重要调节作用。
[Abstract]:Thymosin 尾 4 (Thymosin 尾 4T 尾 -4) is an actin chelating molecule with multiple biological functions in eukaryotic cells. In this study, the full-length cDNA sequence of T 尾 -4 gene was cloned successfully from the full-length cDNA library of deer antler tip tissue in sika deer for the first time, and the amino acid sequence of this gene was analyzed by bioinformatics. The results showed that the full-length cDNA of T 尾 -4 gene of sika deer was 770bp, encoding 44 amino acids, the relative molecular weight was 5 052.65 u, the theoretical isoelectric point was 5.02, and the proportion of lysine and glutamate residues in the primary structure was the highest. The main components of secondary structure are 伪-helix and irregular crimp. The amino acid sequence of T 尾 -4 protein contained "Thymosin family" domain, no N-terminal signal peptide and no transmembrane region. Homologous sequence analysis showed that sika deer was closely related to gibbon, human and cattle, and the amino acid homology was higher than 85.T 尾 -4 in the full-length cDNA library of pilose antler tip tissue. These results suggest that it may play an important role in the growth and development of velvet antler.
【作者单位】：东北林业大学;
【基金】：国家自然科学基金资助项目(31271324) 中央高校基本科研业务费专项资金资助(2572014EA05-03;2572015CA18)
【分类号】：S825

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