基于转录组分析的大针茅响应羊啃食的基因表达

发布时间：2018-07-08 12:14

本文选题：大针茅 + 羊啃食　；参考：《中国草地学报》2017年03期

【摘要】：利用新一代高通量测序手段对大针茅受到羊啃食后2h的实验组与未啃食的对照组叶片进行Illumina平台的转录组测序(RNA sequences),共得到147561个序列读取片段(reads),组装成64738条单基因簇(Unigenes),Unigenes平均长度为1367.21bp,N50为2017bp。随机挑选11个基因利用实时荧光定量PCR对转录组数据库进行了验证。基于转录组数据分析淀粉和蔗糖代谢通路中关键调控基因表达差异,结果表明:随机挑选的11个基因的表达差异变化与转录组数据上下调趋势一致,在淀粉与蔗糖代谢通路中相关基因的表达差异变化利于淀粉、蔗糖的积累。大针茅在羊啃食后淀粉与蔗糖含量的增加有利于结实,对后代的传播意义重大。
[Abstract]:A new generation of high-throughput sequencing method was used to sequence the Illumina platform of the experimental group and the ungnawed control leaf 2 hours after the fescue was gnawed by sheep. (reads), sequences), obtained 147561 read fragments (reads), was assembled into 64738 single genes. The average length of Unigenes is 1367.21bpN _ (50), which is 2017bp _ (50). Eleven genes were randomly selected to verify the transcriptional database by real-time fluorescent quantitative PCR. Based on transcriptional data, the differences of expression of key regulatory genes in starch and sucrose metabolic pathways were analyzed. The results showed that the differences in expression of 11 genes selected at random were consistent with the down-regulation trend in transcriptional data. The difference in the expression of related genes in the pathway of starch and sucrose metabolism is beneficial to the accumulation of starch and sucrose. The increase of starch and sucrose content in fescue after sheep gnawing is beneficial to seed bearing and is of great significance to the transmission of offspring.
【作者单位】：中国农业科学院草原研究所/农业部草地生态与修复治理重点实验室;内蒙古农业大学生命科学学院;
【基金】：内蒙古自然科学基金项目(2015MS0305) 中国科学院西部之光“人才培养”项目中国农业科学院草原研究所科技创新工程资助
【分类号】：S543.9

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