鸽SPAG6基因多态性与精子活力、体尺性状的相关性研究

发布时间：2018-07-08 13:11

本文选题：鸽 + 精子相关抗原蛋白(SPAG)　；参考：《中国畜牧杂志》2017年05期

【摘要】：为探讨鸽精子相关抗原6蛋白(SPAG6)基因单核苷酸多态性(SNPs)及其与鸽精子活力和体尺性状之间的关系,选择98只12月龄银王鸽,采用DNA直接测序法对SPAG6基因部分片段进行SNPs筛选。结果表明:在SPAG6基因编码区和非编码区分别发现2个和7个突变位点,其中G26789T位点BB基因型个体精子活力显著高于AB基因型(P0.05),AA基因型个体胫长均值显著高于BB基因型(P0.05);C16181G和C16336T位点与胫围显著相关(P0.05),BB基因型为优势基因型;T27507C位点AA基因型个体胫围均值显著高于AB基因型(P0.05);T30364C位点3种基因型个体胸宽差异显著,其中BB基因型为优势基因型(P0.05)。由此可见,SPAG6基因与精子活力和体尺性状密切相关,可以作为银王鸽精子活力与体尺性状分子标记的候选基因。
[Abstract]:To investigate the single nucleotide polymorphisms (SNPs) of the spermatozoa associated antigen 6 (SPAG6) gene in pigeon and its relationship with the sperm motility and body size traits of pigeon, 98 12 month old silver pigeons were selected and selected SNPs for SPAG6 gene by DNA direct sequencing. The results showed that two and seven mutation sites were found in the coding and non-coding regions of SPAG6 gene, respectively. The sperm motility of BB genotype at G26789T locus was significantly higher than that of AB genotype (P0.05) the average tibial length of AA genotype was significantly higher than that of BB genotype (P0.05). The mean tibial circumference of individuals was significantly higher than that of AB genotypes (P0.05). BB genotype was the dominant genotype (P0.05). Therefore, SPAG6 gene is closely related to sperm motility and body size traits, and can be used as a candidate gene for sperm motility and body size traits in silver pigeon.
【作者单位】：浙江省农业科学院畜牧兽医研究所;南京农业大学动物科技学院;浙江省畜牧技术推广总站;
【基金】：浙江省农业科学院地方科技合作项目(WZ20140002) 重点科学技术研究开发计划(GKZ101503) 湖州市攻关计划农业项目(2016GN07)
【分类号】：S836

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