STING基因荧光定量检测方法的建立及其在鸭组织中的分布

发布时间：2018-07-08 15:27

  本文选题：鸭 + STING基因　； 参考：《畜牧与兽医》2017年07期

【摘要】：根据鸭STING基因预测序列,设计了2对特异性引物,通过试验条件摸索,首次建立了基于SYBR Green荧光定量PCR检测鸭STING基因方法,并使用该方法对STING在鸭不同组织中的含量进行检测。结果:本研究建立的检测方法,能检测低至4.8×10~3拷贝数/μL的样品;其标准曲线线性范围是3.5×10~(-5)~0.27 ng/μL,具有良好的重复性。组织分布研究发现,STING在腺胃中表达量最高,在气管、肺脏和小肠中表达量较高,在脾、肾、法氏囊、肌胃、肝、盲肠呈中等表达,在肌肉和皮肤中表达量最低。本研究成功建立了鸭STING基因的荧光定量PCR检测方法,为深入研究STING基因在鸭抗病毒感染中的作用奠定了基础。
[Abstract]:According to the prediction sequence of duck STING gene, 2 pairs of specific primers were designed. The method of detecting duck STING gene based on SYBR Green fluorescence quantitative PCR was established for the first time. The method was used to detect the content of STING in different tissues of ducks. Results: the detection method established in this study can detect a copy of 4.8 x 10~3 copies. The linear range of the standard curve is 3.5 * 10~ (-5) ~0.27 ng/ mu L and has good repeatability. The tissue distribution study found that the expression of STING in the stomach is the highest, the expression in the trachea, the lungs and the small intestine is high, and the expression in the spleen, kidney, bursa of the bursa, the stomach, the liver and the cecum is the lowest. This study is the lowest in the muscles and the skin. The fluorescence quantitative PCR detection method of duck STING gene was successfully established, which laid a foundation for further research on the role of STING gene in duck virus infection.
【作者单位】： 上海交通大学农业与生物学院/上海市兽医生物技术重点实验室;上海市宝山区动物疫病预防控制中心;
【基金】：国家自然科学基金(31672524)
【分类号】：S834
，

本文编号：2107872