大豆GmFDL06基因抗干旱及耐盐性研究

发布时间：2018-07-08 16:37

  本文选题：bZIP转录因子 + GmFDL　； 参考：《大豆科学》2017年03期

【摘要】：bZIP转录因子在植物防卫反应和逆境胁迫应答过程中起着十分重要的作用,大豆GmFDL06是bZIP转录因子家族A组成员,其表达水平受PEG和高盐胁迫影响。本研究分析了GmFDL06转基因大豆苗期的抗干旱和耐盐性。PEG和盐处理后GmFDL06转基因植株的相对植株高度(RPH)和相对植株干重(RSDW)显著高于非转基因大豆东农50。在盐胁迫下,GmFDL06转基因植株比东农50伤害程度低,并且GmFDL06过表达减少了Na~+离子积累,降低了盐胁迫带来的伤害,且过表达GmFDL06促进了下游逆境胁迫基因的表达。这些研究结果表明GmFDL06参与大豆非生物胁迫反应,并有潜力改善大豆的干旱和盐胁迫耐受性,为大豆抗逆基因的研究及抗逆大豆材料的筛选提供了依据。
[Abstract]:BZIP transcription factor plays an important role in plant defense response and stress response. Soybean GmFDL06 is a member of bZIP transcription factor family A, and its expression level is affected by PEG and high salt stress. The drought and salt tolerance of GmFDL06 transgenic soybean seedlings. PEG and relative plant height (RPH) and relative plant dry weight (RSDW) of GmFDL06 transgenic soybean after salt treatment were significantly higher than those of non-transgenic soybean Dongnong 50. The damage of GmFDL06 transgenic plants under salt stress was lower than that of Dongnong 50, and the overexpression of GmFDL06 decreased the Na ~ + accumulation and the damage caused by salt stress, and the overexpression of GmFDL06 promoted the expression of downstream stress genes. These results indicated that GmFDL06 was involved in the abiotic stress response of soybean and had the potential to improve the tolerance of soybean to drought and salt stress, which provided a basis for the study of soybean stress resistance genes and the screening of stress resistant soybean materials.
【作者单位】： 哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室;中国科学院东北地理与农业生态研究所/大豆分子育种实验室;
【基金】：国家自然科学基金(31071445,31171579,31201222和31371643) 转基因生物新品种培育重大专项(2016ZX08004-002-003)
【分类号】：S565.1
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本文编号：2108053