不同葡萄糖水平对C2C12细胞线粒体能量代谢相关基因表达影响的研究

发布时间：2018-07-08 20:59

本文选题：CC细胞 + 葡萄糖水平　；参考：《基因组学与应用生物学》2017年11期

【摘要】：本实验目的是通过细胞实验检测葡萄糖水平对线粒体能量代谢相关基因(PGC-1α,ERRα,NRF-1,TFAM和线粒体基因)表达的影响,为研究相关调控基因在2型糖尿病中所起到的作用打下基础。细胞实验通过用两种不同葡萄糖水平的培养基对C2C12细胞进行培养,QRT-PCR检测线粒体能量代谢关键调控基因表达水平。结果显示,细胞培养过程中PGC-1α、ERRα、NRF-1、TFAM和线粒体基因(12S r RNA和COX2)表达变化趋势基本一致,60 h高糖组的基因相对表达量高于低糖组。在C2C12肌细胞中,适度的葡萄糖能诱导PGC-1α及其下游基因ERRα、NRF-1、TFAM的表达水平上升,增加mt DNA数量,促进肌细胞能量代谢,提高对糖的利用能力。
[Abstract]:The purpose of this study was to investigate the effect of glucose level on the expression of mitochondrial energy metabolism-associated genes (PGC-1 伪 -ERR 伪 -NRF-1TFAM and mitochondrial genes) by cell experiments, and to lay a foundation for the study of the role of related regulatory genes in type 2 diabetes. C2C12 cells were cultured in two different glucose culture medium in cell experiment to detect the expression level of mitochondrial energy metabolism key regulatory gene by QRT-PCR. The results showed that the relative expression of PGC-1 伪 -ERR 伪 -NRF-1TFAM and mitochondrial gene (12S r RNA and COX2) in high glucose group at 60 h was higher than that in low glucose group. In C2C12 myocytes, moderate glucose could increase the expression level of PGC-1 伪 and its downstream gene err 伪 NRF-1 TFAM, increase the number of Mt DNA, promote energy metabolism of myocytes, and enhance the utilization of glucose.
【作者单位】：广西大学动物科学技术学院;防城港市动物疫病预防控制中心;
【基金】：国家自然科学基金项目(81360135) 广西自然科学基金项目(2013GXNSFAA019187)共同资助
【分类号】：R587.1

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