花生花青素合成相关基因的表达与种皮颜色关系

发布时间：2018-07-10 17:42

本文选题：花生品种 + 种皮颜色　；参考：《中国油料作物学报》2017年05期

【摘要】：为了探讨花青素合成相关基因表达与种皮颜色的关系,本文采用HPLC和实时荧光定量PCR技术,分析4种不同种皮颜色的花生品种种皮花青素的种类和含量,以及花青素合成相关基因在4个品种5个荚果发育关键时期的差异表达。结果表明:花生种皮的花青素主要由飞燕草色素、矢车菊色素和天竺葵色素等三种色素组成;花生种皮的外观颜色和深浅主要由飞燕草色素和矢车菊色素含量决定,飞燕草色素和矢车菊色素含量越高,种皮颜色越深。花青素合成相关基因主要在种子充实期(开花后约40~50d)上调表达。花生种皮颜色的深浅与查耳酮合成酶基因(CHS)、查耳酮异构酶基因(CHI)、二氢黄酮醇4-还原酶基因(DFR)、类黄酮-3'羟化酶基因(F3'H)和花色苷合成酶基因(ANS)等基因在种子充实期高表达显著相关。上述5个基因表达量愈高,种皮颜色愈深。
[Abstract]:In order to investigate the relationship between anthocyanin biosynthesis related gene expression and seed coat color, HPLC and real-time fluorescence quantitative PCR were used to analyze the species and content of anthocyanins in 4 peanut cultivars with different coat colors. And the differentially expressed genes related to anthocyanin biosynthesis in 4 cultivars and 5 pods at critical stages of development. The results showed that the anthocyanins of peanut seed coat were mainly composed of three kinds of pigments, such as swallows, cornflower and geranium pigments, and the appearance color and depth of peanut seed coat were mainly determined by the content of swallows pigment and cornflower pigment. The higher the pigment content of swallow and cornflower, the darker the color of seed coat. Anthocyanin biosynthesis related genes were mainly upregulated at the filling stage (about 40 ~ 50 days after anthesis). The color of peanut seed coat was significantly correlated with the high expression of Chalcone Synthase (CHS), Chalcone Isomerase (Chi), Dihydroflavone 4-Reductase (DFR), flavonoid 3 'Hydroxylase (F3H) and anthocyanin Synthase (ans). The higher the expression of the above 5 genes, the darker the color of seed coat.
【作者单位】：广东省农业科学院作物研究所广东省农作物遗传改良重点实验室;
【基金】：国家自然科学基金(31501246) 广东省科技计划项目(2015B020231006;2015A020209051;2016B020201003)
【分类号】：S565.2

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