基于PCR Array技术探讨健脾祛痰化瘀方对脾虚痰浊动脉粥样硬化巴马小型猪主动脉AS信号通路相关基因表达的影响

发布时间：2018-07-11 14:22

  本文选题：健脾祛痰化瘀方 + 动脉粥样硬化　； 参考：《中华中医药学刊》2017年10期

【摘要】：目的:利用PCR Array技术观察健脾祛痰化瘀中药对脾虚痰浊动脉粥样硬化(AS)巴马小型猪主动脉AS信号通路相关基因表达变化的影响,探讨健脾祛痰化瘀方防治AS的可能机制。方法:将15只巴马小型猪随机分为正常组、模型组和治疗组,每组5只,正常组普通饲料喂养,模型与治疗组给予高热量高脂饲料喂养并进行冠脉球囊挤压伴拉伤及跑步措施进行干预至24周,制备巴马小型猪病症结合脾虚痰浊AS模型,治疗组除上述造模措施外给予健脾祛痰化瘀方灌胃24周。呼吸机麻醉后取材,提取各组主动脉总RNA,应用AS信号通路PCR芯片检测健脾祛痰化瘀方对脾虚痰浊AS巴马小型猪主动脉AS信号通路相关基因mRNA表达变化的影响。结果:PCR Array结果显示:与空白组相比,模型组显著上调的基因共13个,如ACE、BAX、SELE等基因,显著下调的基因共16个,如BCL2A1、LDLR、LPL等基因;与模型组相比,治疗组显著上调的基因共14个,如BCL2A1、LDLR、BCL2等,显著下调的基因共13个如BAX、PTGS1、SOD1等。结论:健脾祛痰化瘀方可能参与调控主动脉AS信号通路中脂质代谢与凋亡等相关基因表达从而起到防治脾虚痰浊AS巴马小型猪AS形成及稳点斑块的作用。
[Abstract]:Objective: to observe the effect of traditional Chinese medicine for invigorating spleen and removing phlegm and removing blood stasis on the expression of gene related to as signal pathway in aorta of Bama miniature pigs with spleen deficiency and phlegm turbid atherosclerosis (as), and to explore the possible mechanism of Jianpi Quphlegm Huayu recipe in preventing and treating as. Methods: fifteen Bama miniature pigs were randomly divided into normal group, model group and treatment group with 5 in each group. The model and treatment group were fed with high-calorie and high-fat diet and were treated with coronary balloon extrusion with tension and running for 24 weeks to establish the model of as with spleen deficiency and phlegm turbidity in Bama mini-pigs. The treatment group was treated with Jianpi, phlegm and blood stasis decoction for 24 weeks. After ventilator anesthesia, the aorta total RNAs were extracted, and the effects of Jianpi Quphan Huayu recipe on the mRNA expression of as signal pathway related gene in as mini-pig aorta were detected by as signal pathway PCR chip. Results compared with the control group, 13 genes were significantly up-regulated in the model group and 16 genes were down-regulated, such as BCL2A1, LDLRLPL, and 14 genes were significantly up-regulated in the treatment group compared with the control group. For example, BCL2A1, LDLRN, BCL2 and so on, 13 genes were significantly down-regulated, such as BAXA PTGS1, SOD1 and so on. Conclusion: Jianpi Quphan Huayu recipe may be involved in regulating the expression of related genes such as lipid metabolism and apoptosis in aortic atherosclerosis signaling pathway and thus play a role in preventing and treating as with spleen deficiency and phlegm turbidity and stabilizing plaque.
【作者单位】： 辽宁中医药大学中医脏象理论及应用教育部重点实验室;辽宁省中医转化医学研究中心;辽宁中医药大学附属医院;
【基金】：国家重点基础研究发展计划(“973”计划)项目(2013CB531704) 辽宁省高等学校优秀人才支持计划项目(LR2015041)
【分类号】：R285.5
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本文编号：2115485