绞缢对苹果矮砧压条新梢激素含量及生根相关基因表达的影响

发布时间：2018-07-11 13:29

本文选题：苹果 + 矮化砧木　；参考：《园艺学报》2017年04期

【摘要】：以1年生苹果矮化砧木‘9-3’为试材,研究了绞缢处理对压条新梢生根和生根过程中内源激素含量及生根相关基因表达的影响。结果表明:绞缢处理的新梢生根时间早于对照,且其生根率显著高于对照;在愈伤组织形成期,绞缢处理的新梢内IAA、ABA、ZR含量和IAA/ABA、IAA/GA_3、IAA/ZR比值均显著高于对照,ARRO-1、ARF7和ARF19基因表达量也显著高于对照;在不定根发生始期,绞缢处理的新梢内IAA和GA_3含量及ARRO-1基因的表达量均显著高于对照;在不定根发生盛期,绞缢处理的新梢内IAA含量和IAA/ABA、IAA/GA_3、IAA/ZR比值均显著低于对照,而ZR含量和ARRO-1基因表达量均显著高于对照。
[Abstract]:The effects of twisting and hanging on endogenous hormone content and rooting related gene expression in rooting and rooting of stumped shoots were studied using 1-year-old apple dwarf rootstock as test material. The results showed that the rooting time of the new shoot was earlier than that of the control, and the rooting rate of the new shoot was significantly higher than that of the control, and at the stage of callus formation, The content of IAA AABAZR and the ratio of IAA / ABAN IAA / GA3 / ZR were significantly higher than those of the control group, and the expression of IAA, GADA 3 and ARRO-1 gene were significantly higher than those of the control at the beginning of adventitious root formation, and the contents of IAA, GADA 3 and ARRO-1 gene in the new shoot were significantly higher than those in the control group at the beginning of the formation of adventitious roots in the new shoot of the strangulation treatment, and the expression of the ARRO-1 gene in the new shoot was significantly higher than that in the control group at the beginning of adventitious root formation. At the peak of adventitious root formation, IAA content and IAA / ABA- IAA / GA3IAA / ZR ratio were significantly lower than those of control, but ZR content and ARRO-1 gene expression were significantly higher than those of control.
【作者单位】：河北农业大学园艺学院;
【基金】：国家现代农业产业技术体系建设专项资金项目(CARS-28) 公益性行业(农业)科研专项子课题(201203075-05) 河北省科技厅资助项目(16226312D-7)
【分类号】：S661.1

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