甘薯IbFBA5基因的时空表达及与碳水化合物积累的关系

发布时间：2018-07-11 18:29

本文选题：甘薯 + 果糖-　；参考：《植物生理学报》2017年05期

【摘要】：果糖-1,6-二磷酸醛缩酶(FBA)是糖酵解、糖异生、磷酸戊糖途径及光合作用过程的关键酶。本研究利用荧光定量分析研究 IbFBA5基因在甘薯不同组织及不同生长时期的表达及与叶片和贮藏根中碳水化合物积累的关系。结果表明, IbFBA5具有组织表达特异性,在甘薯‘徐781’叶片中表达量最高,其次是地下部(牛蒡根、储藏根和纤维根),叶柄、茎和花中表达量较低;甘薯储藏根初始膨大期至膨大盛期, IbFBA5在‘徐薯18’叶片中表达量均极显著高于在‘徐781’中的表达量;而在储藏根初始膨大期和膨大盛期,该基因在‘徐781’储藏根中的表达量均显著或极显著高于‘徐薯18’中的表达量。可溶性糖和淀粉含量分析表明,甘薯初始膨大期至膨大中期,叶片和储藏根中果糖和葡萄糖的含量均极显著提高,储藏根中淀粉含量也极显著提高,然而储藏根中的蔗糖含量及叶片中淀粉含量极显著降低。甘薯叶片和储藏根中 IbFBA5的表达模式分别与不同时期叶片中淀粉的含量变化和储藏根中果糖及葡萄糖的含量变化趋势具有一致性。
[Abstract]:Fructose 6 diphosphate aldolase (FBA) is a key enzyme in glycolysis, glycosylation, pentose phosphate pathway and photosynthesis. In this study, fluorescence quantitative analysis was used to study the expression of IbFBA5 gene in different tissues and growth stages of sweet potato and the relationship between IbFBA5 gene expression and carbohydrate accumulation in leaves and storage roots. The results showed that IbFBA5 had tissue expression specificity, which was the highest in sweet potato 'xu781' leaves, followed by underground (burdock root, storage root and fibrous root), lower in petiole, stem and flower. The expression of IbFBA5 in the leaves of 'Xushu 18' was significantly higher than that in' Xu781'from the initial expansion stage of storage root to the peak stage of the storage root, but the expression level of IbFBA5 in the leaves of 'Xushu 18' was significantly higher than that in the' Xu781'leaf, but in the initial expansion stage and the blooming stage of the stored root, The expression of this gene in the stored roots of 'Xu781' was significantly higher than that in 'Xushu 18'. The analysis of soluble sugar and starch content showed that the contents of fructose and glucose in leaves and storage roots were significantly increased from the initial expansion stage to the middle stage of the expansion, and the starch content in the stored roots was also significantly increased. However, the content of sucrose in storage roots and starch content in leaves decreased significantly. The expression patterns of IbFBA5 in sweet potato leaves and storage roots were consistent with the changes of starch content in leaves and fructose and glucose contents in storage roots.
【作者单位】：江苏徐淮地区徐州农业科学研究所/农业部甘薯生物学与遗传育种重点实验室/江苏徐州甘薯研究中心;中国农业科学院甘薯研究所;
【基金】：现代农业产业技术体系建设专项资金(CARS-11,甘薯) 国家高技术研究发展计划(2012AA101204) 江苏省重点研发计划项目(BE2015313)~~
【分类号】：S531

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