水杨酸和脯氨酸对盐胁迫下生菜相关基因表达的影响

发布时间：2018-07-11 18:37

本文选题：盐胁迫 + 生菜　；参考：《浙江农业学报》2017年09期

【摘要】：为了探明水杨酸(SA)和脯氨酸(Pro)在缓解植物盐胁迫中的作用,以生菜品种‘美国大叶速生’为材料,采用营养液水培方式,分别使用盐胁迫水培液以及盐胁迫水培液中分别添加SA和Pro 2种外源物质处理生菜幼苗,并且测定相关形态指标、生理指标以及处理后与盐胁迫逆境相关基因的表达量。研究表明,与对照相比,在盐胁迫下加入0.2 mmol·L~(-1)SA和0.2 mmol·L~(-1)Pro处理24 h时,生菜的形态指标缓解效果明显。半定量PCR与荧光定量PCR研究结果基本一致,同时相关抗逆基因LSNCED1、LSNCED2、LSP5CS的表达量在盐胁迫下都有不同程度的上升。结果表明,添加0.2 mmol·L~(-1)SA和0.2 mmol·L~(-1)Pro处理24 h能够缓解盐胁迫对生菜造成的伤害。
[Abstract]:In order to find out the role of salicylic acid (SA) and proline (Pro) in alleviating plant salt stress, the lettuce variety 'American large Leaf Fast-growing' was used as the material, and the nutrient solution was used for hydroponic cultivation of salicylic acid (SA) and proline (Pro). The seedlings of lettuce were treated with salt-stress hydroponics and salt-stress hydroponics with SA and Pro, respectively, and the relative morphological indexes, physiological indexes and the expression of genes related to salt stress were measured. The results showed that compared with the control, the relief effect of lettuce morphological index was obvious when 0.2 mmol L ~ (-1) SA and 0.2 mmol L ~ (-1) Pro were added under salt stress for 24 h. The results of semi-quantitative PCR and fluorescence quantitative PCR were basically consistent, and the expression of LSNCED1 / LSNCED2LSP5CS was increased in different degree under salt stress. The results showed that adding 0.2 mmol L-1 SA and 0.2 mmol L-1 Pro for 24 h could alleviate the injury caused by salt stress on lettuce.
【作者单位】：江苏农林职业技术学院;江苏现代园艺工程技术中心;睢宁县高作镇农业技术推广服务中心;
【基金】：江苏省农业三新工程(SXGC[2015]311) 江苏高校品牌专业建设工程资助项目(PPZY2015B173) 青蓝工程
【分类号】：S636.2

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