凡纳滨对虾养殖期间生长性能、生长相关基因及生长标记的初步探究

发布时间：2018-07-11 21:01

本文选题：凡纳滨对虾 + 幼虾　；参考：《上海海洋大学》2017年硕士论文

【摘要】：为探究凡纳滨对虾养殖2-3月龄阶段的生长差异、生长相关基因及生长标记,本实验通过采集对虾生长第28、38、48、58、68、73天的凡纳滨对虾,共取样857只。按照生长速率不同将体重排名的前10%(14只)、中间10%(14只)、最后10%(14只)分为生长速率高中低三组。根据统计量用幂函数W=aLb进行体长体重生长关系的拟合;体长体重取对数,确定所有样品在内及不同生长速率组最佳直线方程;同时计算不同生长速率组对虾的绝对日增长、绝对日增重和相对日增长率、相对日增重率,以探究不同生长速率组对虾的生长差异。通过2-ΔΔCT方法对不同生长速率组不同生长时期的样品进行RPL18、SNRPG、RPL7基因相对表达量的分析。最后以28天的样品对多态性较好的15对微卫星引物进行体重与位点相关性的分析。结果发现:首先,相同生长时期的三组虾生长速率显著差异(P95%),将这些生长速率不同的虾作为一个整体来分析虾的生长情况可能会出现一定偏差。其次,体长与体重的回归结果为:幂函数W所有样品=1χ10-5L3.0051,直线方程y所有样品=3x-4.92,不同生长速率组的直线方程分别为y高=3.41x-5.71,y中=3.21x-5.30,y低=2.92x-4.78;直线方程中斜率与生长速率呈正相关,三组线性回归方程能更加确切地反映出三组虾的真实生长情况;同时发现,不同生长速率虾的绝对生长(绝对日增长和绝对日增重)符合对虾的实际生长情况,而相对生长(相对日增长率和相对日增重率)与对虾的实际生长情况相反,建议以后分析对虾生长特性时参数选择绝对生长值更为适宜。对于表达量的研究表明,不同生长速率组RPL18、SNRPG基因的相对表达量差异不显著(P0.05),但除28天外,均表现为高速率组低速率组;RPL7基因相对表达量与体长的相关性高于体重,并且均表现为低速率组高中速率组,推测RPL7基因极大可能的参与凡纳滨对虾生长的负调控。本研究得出与体重存在相关性的TUMXLV 10.284微卫星引物,结果表明BB基因型为高速率组和中速率组中的优势基因型(平均体重:3.39±0.37g、2.54±0.30 g),AC、CC为低速率组中的优势基因型(平均体重:0.53±0.10g、0.98±0.3g);所有样品在内的数据显示BB、AB基因型对应的体重(2.85±0.31 g、2.73±0.51g)最高,AC、CC基因型对应的体重(1.18±1.12 g、1.45±0.36g)最低,且体重差异显著(P0.05)。并且发现带B基因型的个体体重均较高,带C基因型的个体体重均较低,且差异显著(P0.05)。
[Abstract]:In order to investigate the growth difference, growth related genes and growth markers of Penaeus vannamei cultured in 2-3 months of age, 857 prawns were collected from the 28 ~ (38) ~ (th) ~ (48) ~ (th) ~ (-) day of the growth of Penaeus vannamei (P.vannamei) in this experiment. According to the growth rate, the top 10% (14), the middle 10% (14) and the last 10% (14) were divided into three groups. According to the statistics, the relationship between body length and body weight was fitted by power function Wlb; the logarithm of body weight was taken to determine the best linear equation for all samples and groups of different growth rates; and the absolute daily growth of Penaeus penaeus at different growth rates was calculated. Absolute daily gain, relative daily growth rate and relative daily gain rate were used to explore the growth differences of shrimp in different growth rates. The relative expression of RPL18, SNRPGN RPL7 gene in different growth rate groups was analyzed by 2- 螖 CT. At last, 15 pairs of microsatellite primers with good polymorphism were used to analyze the correlation between body weight and locus. The results showed that: first, the growth rate of the three groups of shrimp in the same growth period was significantly different (P95%). Secondly, the regression results of body length and body weight were as follows: power function W all samples 蠂 ~ (-1) X ~ (-5) L ~ (3.0051), linear equation y _ y all samples ~ (3x-4.92), the linear equations of different growth rate groups were y ~ (3.41) -5.71 ~ (-1) y low 2.922x-4.78, and the slope of the linear equation was positively correlated with growth rate. Three groups of linear regression equations can more accurately reflect the actual growth of the three groups of shrimp, and found that the absolute growth (absolute daily growth and absolute daily gain) of shrimp at different growth rates is in line with the actual growth of Penaeus Monodon. However, the relative growth (relative daily growth rate and relative daily gain rate) is contrary to the actual growth of Penaeus Monodon. It is suggested that it is more appropriate to choose the absolute growth value when analyzing the growth characteristics of Penaeus penaeus in the future. The results showed that there was no significant difference in the relative expression of RPL18SN-SNRPG gene among different growth rate groups (P0.05), but the correlation between the relative expression of RPL7 gene and body length was higher than that of body length in high rate group and low rate group, except for 28 days. The RPL7 gene may be involved in the negative regulation of the growth of Penaeus vannamei. In this study, the microsatellite primers TUMXLV 10.284 correlated with body weight were obtained. The results showed that BB genotype was the dominant genotype in high rate group and middle rate group (mean weight: 3.39 卤0.37 g / 2.54 卤0.30 g). ACCC was the dominant genotype in low rate group (mean weight was 0.53 卤0.10 g / 0.98 卤0.3 g), and all samples showed that the weight of BBAB genotype corresponded to that of BBAB genotype. (2.85 卤0.31 g / L 2.73 卤0.51g) the highest weight corresponding to the ACCC genotype was the lowest (1.18 卤1.12 g / L 1.45 卤0.36 g). And the weight difference was significant (P0.05). It was found that the individuals with genotype B and genotype C had higher body weight and lower body weight than those with genotype C, and the difference was significant (P0.05).
【学位授予单位】：上海海洋大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S968.22

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