黑曲霉TRK基因家族的全基因组鉴定与分析

发布时间：2018-07-12 16:47

本文选题：黑曲霉 + TRK基因家族　；参考：《基因组学与应用生物学》2017年11期

【摘要】：TRK是一种与钾离子吸收转运相关的基因,在调节生物体生命活动方面起着重要的作用。本研究建立在黑曲霉(Aspergillus niger)基因组数据库基础上,利用生物信息学手段鉴定黑曲霉TRK基因家族成员,分析其基因结构特征和系统进化关系;并利用MEGA 6.06软件,采用最大似然法(MLT)构建系统发育树;使用PAL2NAL软件进行da/ds估算,利用GSDS 2.0软件进行基因结构分析,MEME程序进行Motif分析。在黑曲霉基因组中共鉴定TRK基因家族4个成员:An Trk1、An Trk2、An Trk3、An Trk4,发现均含有TRK特征结构域和保守的Motif基序,不同基因间结构差异较大,但聚类关系较近的基因其结构相似。黑曲霉中TRK基因家族成员在基因结构特征等方面与酵母存在着显著差异,亲缘关系和蛋白互作网络分析进一步验证了这个结论,因此推断黑曲霉中TRK基因还存在其他的调节途径,这个结论为进一步研究黑曲霉TRK渗透胁迫相关蛋白功能、作用机制提供理论依据,从而为了解黑曲霉的渗透调节机理奠定基础。
[Abstract]:TRK is a gene related to the absorption and transport of potassium ions, which plays an important role in regulating biological activities. Based on the (Aspergillus niger) genomic database of Aspergillus Niger, this study identified the members of Aspergillus Niger (Aspergillus niger) gene family by bioinformatics, analyzed its gene structure and phylogenetic relationship, and used MEGA6.06 software. The phylogenetic tree was constructed by maximum likelihood method (MLT), da/ds was estimated by PAL2NAL software, and motif analysis was carried out by GSDS2.0 program. In the genome of Aspergillus Niger, four members of TRK gene family: an Trk1An Trk2An Trk3An Trk3An Trk4 were identified. It was found that TRK characteristic domains and conserved motif were found in all genes. TRK gene family members in Aspergillus Niger have significant differences with yeast in gene structure and other aspects. The analysis of genetic relationship and protein interaction network further verifies this conclusion. Therefore, it is concluded that there are other regulatory pathways of TRK gene in Aspergillus Niger. This conclusion provides a theoretical basis for further study on the function and mechanism of TRK osmotic stress related protein in Aspergillus Niger. So as to understand the mechanism of Aspergillus Niger osmotic regulation laid a foundation.
【作者单位】：江西科技师范大学生命科学学院江西省生物加工过程重点实验室;
【基金】：国家自然科学基金(31171731) 国家863计划(31460447) 江西省赣鄱555英才工程(3000039703) 江西省生物加工重点实验室和体外诊断试剂及仪器协同创新中心共同资助
【分类号】：Q933

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