江苏水稻主导品种的稻瘟病抗性及抗性基因遗传多样性

发布时间：2018-07-12 18:35

本文选题：水稻 + 主导品种　；参考：《湖南农业大学学报(自然科学版)》2017年03期

【摘要】：采用苗期喷雾接种和孕穗期注射接种法,鉴定江苏省49个水稻主导品种的稻瘟病抗性,利用14个抗性基因相关分子标记进行基因型鉴定和遗传多样性分析。结果表明:江苏省水稻主导品种中,未鉴定出高抗苗叶瘟和穗颈瘟的品种,抗、中抗、中感、感和高感苗叶瘟的品种分别有5、7、14、12和11个,抗、中抗、中感、感和高感穗颈瘟的品种分别有2、8、6、9和24个;仅有4个品种对苗叶瘟和穗颈温的抗性均达到中抗及以上水平,占8.2%。14对引物共检测出35个等位变异,多态性信息含量变幅为0.000 0~0.537 5,平均为0.339 8;不同品种出现抗性基因的标记为2~8个,39个品种出现5个以上抗性基因标记(占79.6%)。聚类结果显示,49个品种被聚为3个大类,品种间的相似距离变幅为0.000 0~0.857 1,平均为0.403 9,聚类较为分散,表明种质资源交流频繁,有利基因的共享率较高。
[Abstract]:Blast resistance of 49 dominant rice varieties in Jiangsu Province was identified by spray inoculation at seedling stage and injection inoculation at booting stage. Genotypic identification and genetic diversity analysis were carried out by using 14 molecular markers of resistance genes. The results showed that the cultivars with high resistance to seedling leaf blast and panicle blast were not identified among the dominant rice varieties in Jiangsu Province. The resistance of 4 cultivars to seedling leaf blast and ear neck temperature reached the level of moderate resistance and above, accounting for 8.2dic.14 pairs of primers detected 35 allelic variations. The range of polymorphic information content was 0.000, 0.537 5, with an average of 0.339 8. The markers of resistance genes in different varieties were 2 ~ 8, and more than 5 resistance gene markers were found in 39 varieties (79.6%). The results of clustering showed that 49 varieties were grouped into 3 categories, and the range of similarity distance between varieties was 0.000, 0.857 1, with an average of 0.403 9. The cluster was scattered, indicating frequent exchange of germplasm resources and high sharing rate of favorable genes.
【作者单位】：江苏徐淮地区淮阴农业科学研究所/淮安市农业生物技术重点实验室;嘉兴市农业科学研究院;中国科学院遗传与发育生物学研究所;植物基因组学国家重点实验室;
【基金】：中国科学院战略性先导科技专项(XDA08010401) 浙江省科学技术厅项目(2016C32104) 淮安市应用研究与科技攻关(农业)计划(HAN2015008,HAN201613)
【分类号】：S435.111.41

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