致病疫霉ATP6基因单倍型与地理因素的相关性分析

发布时间：2018-07-14 15:07

【摘要】：致病疫霉(Phytophthora infestans)是马铃薯晚疫病的病原菌,对世界马铃薯的生产造成严重影响。ATP6编码ATP合酶α亚基的第6个亚单位,对ATP合酶的功能及生物的生存生殖至关重要。本研究对来自我国7个群体140株马铃薯晚疫病菌的ATP6基因进行核苷酸序列及其遗传结构和地理因素(海拔、经度和年平均气温)的相关分析。结果表明:7个群体中只有2种单倍型Hapl-1和Hapl-2,其中Hapl-1在群体中所占比例与当地的海拔呈显著正相关,与年平均气温和经度呈显著负相关,而Hapl-2在群体中的比例与当地的海拔、年平均气温和经度的相关正好与Hapl-1相反。群体间的遗传分化度(FST)与群体间直线距离呈显著正相关(r=0.659,P=0.001)。本研究表明,马铃薯致病疫霉ATP6基因单倍型分布和群体地理因素之间具有显著相关关系,该结果为了解致病疫霉的进化趋势和致病疫霉的防治提供了理论依据。
[Abstract]:Phytophthora infestans (Phytophthora infestans) is the pathogen of potato late blight. It has a serious effect on the production of potato in the world. ATP-6 encodes the sixth subunit of ATP-synthase 伪 subunit, which is very important to the function of ATP-synthase and the survival and reproduction of organisms. In this study, the nucleotide sequence, genetic structure and geographical factors (altitude, longitude and annual mean air temperature) of ATPase 6 gene from 140 strains of potato late blight in 7 populations in China were analyzed. The results showed that there were only two haplotypes Hapl-1 and Hapl-2 in the seven populations. The proportion of Hapl-1 in the population was positively correlated with the local altitude, and negatively correlated with the annual mean temperature and longitude, while the proportion of Hapl-2 in the population was significantly correlated with the local altitude. The correlation between annual mean temperature and longitude is the opposite of Hapl-1. The genetic differentiation degree (FST) among populations was positively correlated with the linear distance between populations (r = 0.659). This study showed that there was a significant correlation between the haplotype distribution and population geographical factors of ATP-6 gene in Phytophthora spp. The results provided a theoretical basis for understanding the evolutionary trend of Phytophthora disease and the prevention and control of Phytophthora disease.
【作者单位】：福建农林大学植物病毒研究所/福建省植物病毒学重点实验室;
【基金】：国家马铃薯产业技术体系(编号:CARS-10) 福建省自然科学基金(编号:2016J01111)
【分类号】：S435.32

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