SEPT11基因在小鼠睾丸精子发生过程中的表达特征

发布时间：2018-07-17 16:38

【摘要】：目的初步探讨Septin11(SEPT11)基因在精子发生中的作用。方法通过实时定量PCR、蛋白免疫印迹及免疫荧光等方法检测SEPT11在不同周龄野生型小鼠以及成年睾丸支持细胞激素受体(Ar)特异性敲除小鼠(SCARKO)和Ar全敲除(ARKO)小鼠睾丸中的表达特征。结果 SEPT11基因小鼠出生2周内高表达,随后表达水平降低,出生6周后出现并与未释放的成熟精子共定位且高表达。与野生型小鼠相比,SEPTIN11在SCARKO小鼠和ARKO小鼠睾丸中表达降低(P0.01),m RNA水平显著升高(P0.01);SEPTIN11在成熟精子中定位于尾部。结论 SEPT11基因在小鼠出生时表达最高;SEPTIN11在小鼠成熟精子中定位于尾部;Ar的敲减提高了SEPT11的表达。
[Abstract]:Objective to investigate the role of Septin11 gene in spermatogenesis. Methods the expression of SEPT11 in the testis of wild-type mice and adult testicular Sertoli receptor specific knockout mice (SCARKO) and ar knockout (arko) mice were detected by real-time quantitative PCR, Western blot and immunofluorescence. Results the expression of SEPT11 gene in mice was high within 2 weeks of birth, then decreased. It appeared 6 weeks after birth and co-located and overexpressed with unreleased mature spermatozoa. Compared with wild-type mice, the expression of SEPTIN11 in the testis of SCARKO and ARKO mice decreased significantly (P0.01), and the expression of SEPTIN11 was located in the tail of mature spermatozoa (P0.01). Conclusion the highest expression of SEPT11 gene at birth in mouse spermatozoa is located in mouse mature spermatozoa, and the knockout of ar in the tail of mouse spermatozoa increases the expression of SEPT11.
【作者单位】：广州医科大学研究生院;北京大学深圳医院深圳市男性生殖与遗传重点实验室;
【基金】：深圳市科技计划项目(JCYJ20130402113131202;JCYJ20140415162543017)~~
【分类号】：R698.2

【相似文献】