IL-17A基因多态性及血浆水平与病毒性心肌炎的相关性研究

发布时间：2018-07-17 20:50

【摘要】：目的:探讨白细胞介素17A(interleukin-17A,IL-17A)rs2275913(G-197A)位点单核苷酸多态性(single nucleotide polymorphisms,SNPs)及血浆水平与重庆汉族人群病毒性心肌炎(viral myocarditis,VMC)的关系。方法:收集VMC患者200例,根据病程将患者分为急性期组112例和恢复期组88例,并选择同期200例健康体检的正常人作为对照组,采用聚和酶链反应-限制性内切酶片断长度多态性(PCR-RFLP)、DNA测序等方法检测全部受试者IL-17A基因rs2275913(G-197A)位点SNP,采用酶联免疫吸附法(ELISA)测定两组血清IL-17水平。结果:VMC组IL-17A基因rs2275913(G-197A)多态位点AA基因型和A等位基因频率均显著高于对照组(P0.05),携带AA基因型及携带A等位基因(AA+AG基因型)可增加患VMC的易感性(矫正后ORAA=2.197,95%CIAA=1.208-4.279,PAA=0.003;矫正后ORAA+AG=2.051,95%CIAA+AG=1.134-3.995,PAA+AG=0.009)。VMC组血清IL-17水平明显高于对照组(P0.05),尤以急性期为著(P0.05)。不论是VMC组还是对照组,IL-17A基因rs2275913(G-197A)多态位点中含A等位基因(AA+AG)者血清IL-17水平均显著高于非A等位基因(GG)携带者(P0.01)。结论:IL-17A基因rs2275913位点SNP可能与中国重庆地区汉族人群VMC有关,A等位基因是VMC的易感基因。
[Abstract]:Objective: to investigate the relationship between interleukin-17A (IL-17A) rs2275913 (G-197A) polymorphism (single nucleotide polymorphismsmfSNPs (SNPs) and plasma levels in Chongqing Han population with viral myocarditis (viral). Methods: 200 VMC patients were divided into acute phase group (112 cases) and convalescence group (88 cases) according to the course of disease. Polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) sequencing was used to detect rs2275913 (G-197A) of IL-17A gene in all subjects and serum IL-17 level was measured by enzyme-linked immunosorbent assay (Elisa). Results the frequencies of AA genotype and A allele of IL-17A gene rs2275913 (G-197A) polymorphic locus were significantly higher than those of the control group (P0.05). Carrying AA genotype and A AG genotype increased the susceptibility to VMCs (corrected ORAA 2.1979CIAA 1.208-4.279PAA 0.003 after correction). The level of serum IL-17 in the VMC group was significantly higher than that in the control group (P0.05), especially in the acute phase (P0.05). The serum levels of IL-17 in rs2275913 (G-197A) polymorphic loci with A allele (AA AG) were significantly higher than those in non-A allele (GG) carriers (P0.01). Conclusion the SNPs of the rs2275913 locus of the 1: IL-17A gene may be associated with the allele of VMCs in Chinese Han population in Chongqing area, which may be the susceptible gene of VMCs.
【作者单位】：中国人民武装警察部队重庆市总队医院心血管内科;海南医学院热带病转化医学教育部重点实验室;中国人民武装警察部队重庆市总队医院体检中心;中国人民武装警察部队重庆市总队医院儿科;
【基金】：重庆市科委基础与前沿研究计划项目(No:cstc2015jcyjA10047)
【分类号】：R542.21

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