木豆抗旱相关基因CcGST1克隆与表达分析
发布时间：2018-07-21 11:39
【摘要】：为探讨谷胱甘肽转移酶(GST)的抗逆机理,克隆木豆中的抗旱谷胱甘肽转移酶基因,基于木豆干旱胁迫c DNA文库中1个GST基因相关的EST片段,利用RACE技术从木豆幼苗中克隆了该基因,命名为CcGST1,研究其在不同组织及不同干旱时期的表达特性。结果表明:该基因含1个669 bp的开放阅读框,编码223个氨基酸残基,含有保守的谷胱甘肽-S-转移酶的N端和C端结构域;系统进化树分析结果表明木豆CcGST1与大豆、宽叶菜豆等豆科植物的GST亲缘关系较近;亚细胞定位预测CcGST1蛋白可能定位于叶绿体;荧光定量PCR分析表明,CcGST1在木豆幼苗根、茎和叶中均有表达,但根中的表达水平最高,同时该基因受干旱胁迫诱导表达。据此推测木豆CcGST1基因可能与木豆应激干旱胁迫相关。
[Abstract]:In order to investigate the mechanism of stress resistance of glutathione transferase (GST), the drought-resistant glutathione transferase (DGST) gene was cloned from xylem bean, based on an EST fragment associated with GST gene in c DNA library under drought stress. The gene was cloned from woody bean seedlings by race technique and named CcGST1. The expression characteristics of CcGST1 in different tissues and different drought periods were studied. The results showed that the gene contained an open reading frame of 669 BP, encoding 223 amino acid residues and containing conserved glutathione--Stransferase N-terminal and C-terminal domains. CcGST1 protein may be located in chloroplast by subcellular localization. Fluorescence quantitative PCR analysis showed that CcGST1 was expressed in root, stem and leaf of woody bean seedlings, but the highest expression level was found in root. At the same time, the gene was induced to express under drought stress. It is suggested that the CcGST1 gene may be related to stress drought stress.
【作者单位】： 贵州大学农业生物工程研究院山地植物资源保护与种质创新省部共建教育部重点实验室山地生态与农业生物工程协同创新中心;
【基金】：国家自然科学基金项目(30860224)资助 贵州省农业攻关项目(20092076)资助
【分类号】：Q943.2;S793.9
[Abstract]:In order to investigate the mechanism of stress resistance of glutathione transferase (GST), the drought-resistant glutathione transferase (DGST) gene was cloned from xylem bean, based on an EST fragment associated with GST gene in c DNA library under drought stress. The gene was cloned from woody bean seedlings by race technique and named CcGST1. The expression characteristics of CcGST1 in different tissues and different drought periods were studied. The results showed that the gene contained an open reading frame of 669 BP, encoding 223 amino acid residues and containing conserved glutathione--Stransferase N-terminal and C-terminal domains. CcGST1 protein may be located in chloroplast by subcellular localization. Fluorescence quantitative PCR analysis showed that CcGST1 was expressed in root, stem and leaf of woody bean seedlings, but the highest expression level was found in root. At the same time, the gene was induced to express under drought stress. It is suggested that the CcGST1 gene may be related to stress drought stress.
【作者单位】： 贵州大学农业生物工程研究院山地植物资源保护与种质创新省部共建教育部重点实验室山地生态与农业生物工程协同创新中心;
【基金】：国家自然科学基金项目(30860224)资助 贵州省农业攻关项目(20092076)资助
【分类号】：Q943.2;S793.9
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