NDV分离株F基因序列分析及其部分抗原表位的原核表达
发布时间：2018-07-21 12:02
【摘要】：为了对山东省滨州市周边地区6株新城疫流行情况进行研究,试验对不同毒株的治病力进行测定,采用RT-PCR技术扩增F基因,利用Meg Align软件进行序列分析,同时克隆DZNDV/007株F蛋白的两个抗原表位F72和F161,构建两个融合表达质粒p ET-32a-F72和p ET-32a-F161,并对其进行诱导表达及Western-blot分析。结果表明:5株属于基因Ⅱ型,为弱毒株;1株属于基因Ⅶ型,为强毒株;IPTG诱导得到两个大量表达的融合蛋白His-F72和His-F161,且具有良好的反应原性。说明新城疫基因Ⅶ型毒株已成为我国的主要致病毒株。
[Abstract]:In order to study the epidemic situation of 6 strains of Newcastle disease in the surrounding area of Binzhou City, Shandong Province, the therapeutic power of different strains was tested. The F gene was amplified by RT-PCR and sequenced by Meg Align software. At the same time, two antigenic epitopes F72 and F161of F protein of DZNDV / 007 strain were cloned, two fusion expression plasmids pET-32a-F72 and pET-32a-F161were constructed, and their expression was induced and analyzed by Western-blot. The results showed that the fusion proteins His-F72 and His-F161were induced by IPTG of virulent strain and virulent strain, respectively, and had good reactivity. The results showed that 5 strains belonged to gene type 鈪，
本文编号：2135462
[Abstract]:In order to study the epidemic situation of 6 strains of Newcastle disease in the surrounding area of Binzhou City, Shandong Province, the therapeutic power of different strains was tested. The F gene was amplified by RT-PCR and sequenced by Meg Align software. At the same time, two antigenic epitopes F72 and F161of F protein of DZNDV / 007 strain were cloned, two fusion expression plasmids pET-32a-F72 and pET-32a-F161were constructed, and their expression was induced and analyzed by Western-blot. The results showed that the fusion proteins His-F72 and His-F161were induced by IPTG of virulent strain and virulent strain, respectively, and had good reactivity. The results showed that 5 strains belonged to gene type 鈪，
本文编号：2135462
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