调控铁调素表达的HjV-BMPs-SMADs通路中基因多态性与中国12~18岁青少年贫血的相关性研究

发布时间：2018-07-26 10:37

【摘要】：目的探讨调控铁调素表达的Hj V-BMPs-SMADs通路中基因多态性与中国12~18岁青少年人群贫血的关联性。方法采用整群随机抽样的方法,在四川省成都市、吉林省白城市、广东省肇庆市、贵州省普定县、山东省汶上县、浙江省松阳县共选取657名12~18岁初中一年级寄宿制学生,使用氰化高铁法对其静脉血血红蛋白(Hb)浓度进行检测,并依据《人群贫血筛查方法》对样本进行分类。使用磁珠法提取血液样本中的DNA,经Sequenom Mass Array系统对样本进行29个目标位点的基因多态性检测。将Hb检测分类结果与基因位点分型结果进行卡方检验和t检验,分析该通路中基因多态性与Hb浓度与贫血状况的关系。结果 rs173107的TT纯合子携带人群的贫血风险是GG纯合子携带人群的5.80倍(P0.05,OR=5.80,95%CI 1.34~25.04),Hb浓度分别为(143.7±14.6)和(145.6±12.0)g/L。rs855791的AG杂合子携带人群贫血发生风险是AA纯合子携带人群的4.00倍(P0.05,OR=4.00,95%CI 1.35~11.82),Hb浓度分别为(144.2±14.8)和(145.8±12.0)g/L。G等位基因型携带人群贫血风险是AA纯合子携带人群的3.68倍(P0.05,OR=3.68,95%CI 1.27~10.66),Hb浓度分别为(144.3±14.4)和(145.8±12.0)g/L。两个阳性位点各基因型之间的Hb水平差异均无统计学意义,但可以观察到rs173107的TT纯合子和rs855791的G等位基因携带人群的Hb浓度均低于其他相应基因型组别的Hb浓度。结论 Hj V-BMPs-SMADs通路上的rs173107和rs855791与中国青少年人群贫血之间存在关联。
[Abstract]:Objective to investigate the association of genetic polymorphisms in the Hj V-BMPs-SMADs pathway regulated by fertin expression and anemia in young people aged 12~18 in China. Methods a cluster random sampling method was used to select 657 12 in Chengdu, Jilin, Baicheng, Zhaoqing, Guangdong, Puding County, Shandong Province, Wenshang County, Shandong Province, Songyang County, Zhejiang. ~18 years old junior high school boarding students, using cyanide high iron method to detect the concentration of hemoglobin (Hb) of venous blood, and classify the samples according to the anemia screening method of the crowd. Using the magnetic bead method to extract the DNA in the blood samples, the gene polymorphism of 29 target loci was detected by the Sequenom Mass Array system. Hb The relationship between gene polymorphism and Hb concentration and anemia status in this pathway was analyzed by chi square test and t test. Results the risk of anemia in TT homozygote carrying population of rs173107 was 5.80 times as high as that of GG homozygote (P0.05, OR= 5.80,95%CI 1.34~25.04), and Hb concentration was (143.7 + 14.6), respectively. The risk of anemia in the AG heterozygote carrying population of (145.6 + 12) g/L.rs855791 was 4 times as high as that of the AA homozygote (P0.05, OR=4.00,95%CI 1.35~11.82), and the Hb concentration was (144.2 + 14.8) and (145.8 + 12) g/L.G alleles, the risk of anemia was 3.68 times that of the AA homozygote carrying population (P0.05, OR=3.68,95%CI 1.27~10.66). There was no significant difference in Hb level between Hb concentration (144.3 + 14.4) and (145.8 + 12) g/L. two positive loci, but the Hb concentration of rs173107 TT homozygote and rs855791 G allele was lower than the Hb concentration of other corresponding genotypes. Conclusion r on Hj V-BMPs-SMADs pathway There is a correlation between s173107 and rs855791 and anemia among Chinese youth.
【作者单位】：中国疾病预防控制中心营养与健康所
【基金】：公益性行业科研专项(No.201202012)
【分类号】：R725.5

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