薏苡仁不同组分干预脾虚水湿不化大鼠细胞间隙基因变化

发布时间：2018-07-26 12:10

【摘要】：目的:探讨薏苡仁不同组分对脾虚水湿不化模型大鼠空肠extracellular space组件的影响。方法:以高脂低蛋白饮食+游泳建立脾虚水湿不化大鼠模型,基因芯片技术进行空肠全基因组测定,以Quick-GO注释到的extracellular space组件进行PCA分析。结果:模型组和正常对照组具有非常清楚的差异,模型组主要出现了Sepp1、Lamc2、Csn1s1、Mmp8、Ccl20、Apoe、Apoa5、Pyy、Ccl19、Np4基因的上调和Gnrh1、Col6a3基因的下调,薏苡仁水煎液和各拆分组分均能够对模型大鼠的差异基因进行调节,改善机体微环境,主要起作用的基因是Col6a3、Sepp1、Lamc2、Gnrh1、Myoc、Csn1s1、Mmp8、Ccl20、Arg1、Apoe等。结论:脾虚水湿不化的运化失常应该是以物质由小肠上皮细胞向其他组织器官运送过程的异常为主,薏苡仁各组分的治疗都可使失衡的细胞微环境得到改善。
[Abstract]:Aim: to investigate the effect of different components of Coix seed on jejunal extracellular space in rats with spleen deficiency and dampness. Methods: the rat model of spleen deficiency without dampness was established by high fat and low protein diet and swimming. The whole genome of jejunum was determined by gene chip technique. The PCA was analyzed by extracellular space module annotated by Quick-GO. Results: there was a very clear difference between the model group and the normal control group. In the model group, the expression of Sepp1OLamc2Csn1s1Csn1s1, Ccl20, Apoa5, Apoa5, and the down-regulation of the Gnrh1 and Col6a3 gene were mainly found in the model group. The Coix seed decoction and the separated components could regulate the differentially expressed genes in the model rats. In order to improve the microenvironment, the major genes that play an important role in improving the microenvironment are Col6a3 Sepp1, Lamc2, Gnrh1, Myocus, Csn1s1, Mmp8, Ccl20, Arg1, Apoe, and so on. Conclusion: the abnormal transport of substances from small intestinal epithelial cells to other tissues and organs should be the main reason for the impotence of spleen deficiency and dampness of water. The treatment of Coix seed components can improve the unbalanced microenvironment of cells.
【作者单位】：山东中医药大学中医学院(基础医学院);山东医药技师学院;
【基金】：国家重点基础研究发展计划(973计划)资助项目(No.2013CB531803) 山东省中医药科技发展计划项目(No.2015-008)~~
【分类号】：R285.5

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