苹果抗炭疽菌叶枯病基因SNP和InDel标记的HRM筛选
发布时间：2018-07-31 11:26
【摘要】：以207株‘金冠’与‘富士’苹果的F1杂交分离群体实生树为试材,挑选抗炭疽菌叶枯病基因R_(gls)位点区域内的,第15条染色体上,位于SSR标记S0304673和S0405127之间500 kb物理距离内,由全基因组重测序技术所获得的与抗该病相关的10个SNP及10个InDel标记,通过高分辨率熔解曲线(High Resolution Melting,HRM)技术筛选出与基因R_(gls)位点紧密连锁的2个SNP及4个InDel标记。通过对重组个体的基因型及表型分析,发现标记InDel4227和InDel4254表现出与R_(gls)基因位点共分离,将基因R_(gls)精细定位于标记InDel4199和SNP4299之间100 kb的物理距离内。对该基因位点两侧4.1~4.3Mb距离内的基因进行统计分析,表明在该区段内存在40个有功能注释的基因,涉及到细胞组成、分子功能和生物过程方面共19个GO分类。
[Abstract]:In the F _ 1 hybrid population of 'Jinguan' and 'Fuji' apple, selected the region of resistance to anthrax leaf blight gene R _ (gls), on chromosome 15, located within 500kb physical distance between SSR marker S0304673 and S0405127. Ten SNP and 10 InDel markers associated with the disease resistance were obtained from the whole genome resequencing technique. Two SNP and four InDel markers closely linked to the gene R _ (gls) locus were screened by high-resolution fusion curve (High Resolution melting HRM. By genotypic and phenotypic analysis of the recombinant individuals, it was found that the marker InDel4227 and InDel4254 were coisolated from the R _ (gls) gene locus, and the gene R _ (gls) was located within the physical distance of 100kb between the labeled InDel4199 and SNP4299. The statistical analysis of the genes in the 4.1~4.3Mb distance between the two sides of the gene site showed that there were 40 functional annotated genes in this region, involving 19 go classifications in cell composition, molecular function and biological processes.
【作者单位】： 青岛农业大学园艺学院青岛市园艺植物遗传改良与育种重点实验室;青岛农业大学农学与植物保护学院;青岛农业大学生命科学学院;
【基金】：国家自然科学基金项目(31372032) 国家现代农业产业技术体系建设专项资金项目(CARS-28-01-07) 山东省良种产业化工程项目(620902) “泰山学者”建设工程和青岛市民生科技计划项目(15-9-2-99-nsh,14-2-3-38-nsh) ‘十二五’国家科技支撑计划项目(2013BAD02B01) 山东省优秀中青年科学家科研奖励基金项目(BS2014SW027)
【分类号】：S436.611.1
[Abstract]:In the F _ 1 hybrid population of 'Jinguan' and 'Fuji' apple, selected the region of resistance to anthrax leaf blight gene R _ (gls), on chromosome 15, located within 500kb physical distance between SSR marker S0304673 and S0405127. Ten SNP and 10 InDel markers associated with the disease resistance were obtained from the whole genome resequencing technique. Two SNP and four InDel markers closely linked to the gene R _ (gls) locus were screened by high-resolution fusion curve (High Resolution melting HRM. By genotypic and phenotypic analysis of the recombinant individuals, it was found that the marker InDel4227 and InDel4254 were coisolated from the R _ (gls) gene locus, and the gene R _ (gls) was located within the physical distance of 100kb between the labeled InDel4199 and SNP4299. The statistical analysis of the genes in the 4.1~4.3Mb distance between the two sides of the gene site showed that there were 40 functional annotated genes in this region, involving 19 go classifications in cell composition, molecular function and biological processes.
【作者单位】： 青岛农业大学园艺学院青岛市园艺植物遗传改良与育种重点实验室;青岛农业大学农学与植物保护学院;青岛农业大学生命科学学院;
【基金】：国家自然科学基金项目(31372032) 国家现代农业产业技术体系建设专项资金项目(CARS-28-01-07) 山东省良种产业化工程项目(620902) “泰山学者”建设工程和青岛市民生科技计划项目(15-9-2-99-nsh,14-2-3-38-nsh) ‘十二五’国家科技支撑计划项目(2013BAD02B01) 山东省优秀中青年科学家科研奖励基金项目(BS2014SW027)
【分类号】：S436.611.1
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