骨肉瘤组织差异表达基因筛选及其与患者预后的关系

发布时间：2018-07-31 11:30

【摘要】：目的采用生物信息学的方法筛选骨肉瘤组织差异表达基因,并探讨其与患者预后的关系。方法收集骨肉瘤组织及其配对癌旁正常组织标本各4例份,采用转录组测序技术筛选两种组织中的差异表达基因,并进行基因本体(GO)功能显著性富集分析。在癌症和肿瘤基因图谱骨肉瘤数据库中选择258例份骨肉瘤组织标本,采用Kaplan-Meier曲线分析富集于生物学过程的差异表达基因对患者生存时间的影响。结果骨肉瘤及其癌旁正常组织存在差异表达基因875个,其中表达上调的基因346个、表达下调的基因529个。GO功能显著性富集分析共识别出14个有意义的类型;在6个生物学过程类型中,差异表达基因主要富集于外源性代谢过程、细胞黏附、细胞外基质组成、类固醇代谢过程等;在8个细胞成分类型中,差异表达基因主要富集于细胞外体、细胞外空间、胶原三聚体、核小体等。258例份骨肉瘤组织中,还原酶1(NQO1)基因高表达233例份、低表达25例份,NQO1基因高表达、低表达患者生存时间分别为(2 763±15)、(1 568±13)d,二者比较P0.01;醛脱氢酶3族A1(ALDH3A1)基因高表达233例份、低表达25例份,ALDH3A1基因高表达、低表达患者生存时间分别为(2 725±11)、(1 421±14)d,二者比较P0.01;其余基因表达情况对患者的生存时间均无明显影响(P均0.05)。结论采用生物信息学的方法筛选出骨肉瘤组织差异表达基因875个,主要富集于生物学过程和细胞成分类型;其中NQO1、ALDH3A1基因低表达提示患者预后不良。
[Abstract]:Objective to screen differentially expressed genes in osteosarcoma by bioinformatics and to explore the relationship between differential expression and prognosis of osteosarcoma. Methods four specimens of osteosarcoma and 4 matched normal tissues were collected, and the differentially expressed genes were screened by transcriptome sequencing technique, and the functional enrichment of gene proprioception (GO) was analyzed. Two hundred and fifty-eight specimens of osteosarcoma from cancer and tumor gene map were selected and the effect of differentially expressed genes enriched in biological process on the survival time of patients was analyzed by Kaplan-Meier curve. Results there were 875 differentially expressed genes in osteosarcoma and its adjacent normal tissues, including 346 up-regulated genes and 529 down-regulated genes. A total of 14 significant types were identified by functional enrichment analysis. The survival time of the patients with low expression was (2 763 卤15), (1 568 卤13) d, compared with that of P0.01.The aldehydes dehydrogenase 3 group A1 (ALDH3A1) gene was overexpressed in 233 cases, and the low expression of ALDH3A1 gene was found in 25 cases. The survival time of the patients with low expression was (2 725 卤11), (1 421 卤14) d, compared with that of P0.01.The other gene expression had no significant effect on the survival time of the patients (all P 0. 05). Conclusion 875 differentially expressed genes in osteosarcoma tissues were screened by bioinformatics, which were mainly concentrated in biological processes and cell types, among which the low expression of NQO1 / ALDH3A1 gene suggested poor prognosis in patients with osteosarcoma.
【作者单位】：云南省肿瘤医院;
【分类号】：R738.1

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