多巴胺转运体基因敲除小鼠认知功能的变化

发布时间：2018-07-31 18:13

【摘要】：目的观察多巴胺转运体(DAT)基因敲除小鼠认知功能的变化。方法采用Western印迹及PCR方法检测DAT基因敲除C57BL/6 J小鼠模型,通过对DAT基因敲除小鼠进行Morris水迷宫试验及旷场试验研究小鼠认知能力的变化。结果在Morris水迷宫试验中,与WT组小鼠相比,DAT基因敲除小鼠平均逃避潜伏期明显延长,显示其学习能力下降;在撤掉平台后,其在原平台象限停留时间缩短,表明其记忆能力亦下降。在旷场试验中,与WT组小鼠相比,DAT基因敲除小鼠总的探索路程降低,虽然其自主兴奋性运动增加,但多为无目的性运动,其在中央格停留时间及次数也下降,表明其探索好奇能力明显下降。结论敲除小鼠DAT基因可导致小鼠多动,同时损伤小鼠的认知功能,表明DAT基因可能和学习、记忆、探索能力有关。
[Abstract]:Objective To observe the changes in cognitive function of dopamine transporter (DAT) gene knockout mice. Methods Western blot and PCR method were used to detect the DAT knockout C57BL/6 J mouse model, and the changes in cognitive energy of mice were studied by Morris water maze test and open field test on DAT knockout mice. The results were in Morris water maze test and W. Compared with group T, the average escape latency of DAT gene knockout mice was obviously prolonged and its learning ability decreased. After the withdrawal of the platform, the retention time in the original platform quadrant shortened and its memory ability decreased. In the open field test, the total exploration path of the DAT knockout mice was lower than that of the WT mice, although the spontaneous excitement was independent. The activity of sexual movement increased, but most of it was a purposeful movement, and its retention time and times in the central lattice also decreased, indicating that the ability to explore curiosity was obviously decreased. Conclusion knockout mice DAT gene could lead to mice hyperactivity and damage the cognitive function of mice, indicating that the DAT gene may be related to learning, memory and exploration ability.
【作者单位】：南昌大学第一附属医院精神科;
【分类号】：R749.1

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