Pro-sigmaK inhibitor-BofA基因敲除对嗜热厌氧杆菌SCUT27葡萄糖和木糖利用的影响

发布时间：2018-08-03 12:18

【摘要】：嗜热厌氧杆菌SCUT27(Thermoanaerobacterium aotearoense SCUT27,SCUT27)能同时利用木糖和葡萄糖,是发酵廉价木质纤维素获得乙醇及乳酸的优势菌株。为了提高该菌株对木糖和葡萄糖的利用效率,对其在葡萄糖、木糖和混合糖下不同时间点转录组数据分析的基础上,敲除pro-sigmaK抑制因子BofA(pro-sigmak processing inhibitor BofA,BofA)基因,获得基因工程菌ΔbofA。摇瓶发酵结果显示,与出发菌相比,当以木糖为唯一碳源时,突变株ΔbofA对木糖的代谢速率提高51.43%,乳酸产率提高23.53%;以混合糖(葡萄糖:木糖为=1:1)为碳源时,ΔbofA对木糖的代谢速率提高44.44%,乳酸和乙醇的产率分别提高43.75%和20.00%。由此可见,pro-sigmaK抑制因子BofA是木糖利用的负调控因子,其功能有待进一步研究。
[Abstract]:Anaerobic thermophilic bacteria (SCUT27 (Thermoanaerobacterium aotearoense SCUT27 / SCUT27), which can use xylose and glucose simultaneously, are the dominant strains for the production of ethanol and lactic acid by fermentation of cheap lignocellulose. In order to improve the utilization efficiency of xylose and glucose, on the basis of the analysis of transcriptional data at different time points under glucose, xylose and mixed sugar, the strain of pro-sigmaK inhibitory factor BofA (pro-sigmak processing inhibitor BofA) was knockout and the genetically engineered strain 螖 bofA was obtained. The results of shaking flask fermentation showed that when xylose was the sole carbon source, The metabolic rate of xylose by 螖 bofA was increased by 51.43% and the yield of lactic acid was increased by 23.53%. When mixed sugar (glucose: xylose 1: 1) was used as carbon source, the metabolic rate of 螖 bofA to xylose increased by 44.44%, and the yield of lactic acid and ethanol increased by 43.75% and 20.00%, respectively. Therefore, pro-sigma K inhibitory factor BofA is a negative regulation factor for xylose utilization, and its function needs further study.
【作者单位】：华南理工大学生物科学与工程学院;
【基金】：国家自然科学基金面上项目资助(21676098)
【分类号】：Q78;Q939.9

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