腺苷酸环化酶3缺失下调小鼠嗅觉受体基因表达

发布时间：2018-08-04 09:00

【摘要】：主要嗅觉表皮组织(MOE)是哺乳动物感知气味分子的重要器官,气味诱导是嗅觉受体神经元(ORN)活动的起点,嗅觉受体(OR)结合气味分子后通过环腺苷酸(c AMP)信号通路向下游传递信号。腺苷酸环化酶3(AC3)是此通路中的重要分子。为了探讨AC3缺失对小鼠MOE内ORs基因表达的影响,本文以AC3敲除型小鼠(AC3-/-)和野生型小鼠(AC3+/+)为材料,采用荧光定量PCR(qRT-PCR)、荧光原位杂交(FISH)技术分析了部分ORs基因及与其相关因子在MOE中的表达。qRT-PCR表明,3月龄AC3-/-小鼠MOE中嗅觉受体Olfr15、Olfr16、Olfr533、Olfr536、Olfr1507和Olfr642的表达量均显著下降。出生后PND7、PND30和PND90三个不同发育时期的AC3-/-小鼠MOE原位杂交显示,嗅觉受体Olfr15、Olfr536和Olfr1507表达的细胞数目均减少。进一步qRTPCR分析发现,3月龄AC3-/-小鼠嗅觉受体相关因子Rtp1、Rtp2、Reep1、Lhx2、Emx2和Ric-8b的表达也均发生显著下调。由此推测,AC3缺失导致的ORs及其相关因子的表达下调可能是嗅觉行为障碍的原因之一。
[Abstract]:The main olfactory epidermis (MOE) is an important organ of mammalian sensory odour molecules. Odor induction is the starting point of olfactory receptor neurons (ORN) activity. Olfactory receptor (OR) binds to odour molecules and transmits signals downstream through cyclic adenylate (c AMP) signaling pathway. Adenylate cyclase 3 (AC3) is an important molecule in this pathway. In order to investigate the effect of AC3 deletion on the expression of ORs gene in MOE of mice, AC3 knockout mice (AC3-P -) and wild type mice (AC3 /) were used as materials. Fluorescence quantitative PCR (qRT-PCR) and fluorescence in situ hybridization (FISH) were used to analyze the expression of partial ORs gene and its related factors in MOE. QRT-PCR showed that the expression of olfactory receptor Olfr15, Olfr16, Olfr533, Olfr536, Olfr1507 and Olfr642 in three-month-old AC3-r-mouse MOE was significantly decreased. MOE in situ hybridization of three different developmental stages of PND7, PND30 and PND90 showed that the number of olfactory receptor Olfr15, Olfr536 and Olfr1507 decreased. Further qRTPCR analysis showed that the expression of Rtp1, Rtp2, Rtp2, Rtp2Reep1, Lhx2Emx2 and Ric-8b, was also significantly down-regulated in 3 month old AC3-P / R mice. It is suggested that the down-regulation of ORs and its related factors caused by AC3 deletion may be one of the causes of olfactory behavioral disorders.
【作者单位】：河北大学生命科学学院;河北省生物工程技术研究中心;河北大学预防医学与卫生事业管理系;河北大学医学院;
【基金】：国家自然科学基金(No.31171191,31471178) 河北省自然科学基金(C2016201008) 河北省巨人计划项目(No.201235)资助~~
【分类号】：Q434;Q78

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