褐牙鲆早期发育阶段CRH基因表达及受甲状腺激素的影响

发布时间：2018-08-07 14:41

【摘要】：【目的】探析褐牙鲆仔鱼早期发育阶段促肾上腺皮质激素释放激素基因(CRH)的表达情况及受甲状腺激素的影响作用,为开展牙鲆的人工繁育提供科学依据。【方法】通过实时荧光定量PCR检测褐牙鲆早期发育阶段CRH基因表达量,以ELISA测定褐牙鲆仔鱼甲状腺激素(T3和T4)水平,并测定经外源T3浸泡处理后早期发育褐牙鲆仔鱼CRH基因表达量的变化情况,探究CRH和甲状腺激素在褐牙鲆早期发育阶段的影响及作用关系。【结果】1~8日龄褐牙鲆仔鱼CRH基因相对表达量呈先升高后降低的变化趋势,在5日龄达最高值,显著高于其他日龄的仔鱼(P0.05,下同)。10~42日龄褐牙鲆仔鱼CRH基因主要在其头部表达,尾部CRH基因的相对表达量随着生长发育的进程而逐渐增加。褐牙鲆仔鱼变态前期甲状腺激素T4含量逐渐增加,变态高峰期迅速升高,在变态后期呈下降趋势。较其他早期发育时期,甲状腺激素T3在褐牙鲆变态期维持高水平,但含量低于甲状腺激素T4。22日龄褐牙鲆仔鱼经50 nmol/L外源T3处理2 h后,其头部和尾部CRH基因的相对表达量显著升高;但以外源T3处理8 h后,无论是头部还是尾部,CRH基因的相对表达量均呈显著下降趋势。【结论】褐牙鲆早期变态发育需CRH基因高表达及甲状腺激素积累,二者对褐牙鲆早期变态发育阶段具有重要作用并呈互相代偿效应。
[Abstract]:[objective] to investigate the expression of adrenocorticotropin releasing hormone (CRH) gene in early developmental stage of Paralichthys olivaceus and its effect on thyroid hormone. [methods] the expression of CRH gene in the early developmental stage of Paralichthys olivaceus was detected by real-time fluorescence quantitative PCR, and the thyroid hormones (T3 and T4) were measured by ELISA. The changes of CRH gene expression in the early stage of development of Paralichthys olivaceus were determined after treatment with exogenous T3. To investigate the effect of CRH and thyroid hormone on the early development stage of Paralichthys olivaceus. [results] the relative expression of CRH gene in 8-day-old flounder larvae increased first and then decreased, and reached the highest level at 5 days old. The expression of CRH gene was significantly higher than that of other day-old larvae (P0.05) .1042-day-old. The relative expression of CRH gene in tail increased with the growth and development of the larval flounder, and the relative expression of CRH gene in the tail of the larval flounder was increased gradually with the growth and development of the larval flounder. The content of thyroid hormone T4 in Paralichthys olivaceus increased gradually in the premetamorphosis stage, increased rapidly in the peak period of metamorphosis, and decreased in the late metamorphosis stage. Compared with other early developmental stages, thyroid hormone T3 maintained a high level during the metamorphosis of Paralichthys olivaceus, but its content was lower than that of the larvae treated with 50 nmol/L exogenous T3 for 2 h. The relative expression of CRH gene in the head and tail was significantly increased, but treated with exogenous T3 for 8 h. The relative expression of CRH gene in both head and tail of Paralichthys olivaceus decreased significantly. [conclusion] the high expression of CRH gene and the accumulation of thyroid hormone are required in the early metamorphosis of Paralichthys olivaceus. They play an important role in the early metamorphosis and development of Paralichthys olivaceus.
【作者单位】：上海海洋大学水产与生命学院;
【基金】：国家自然科学基金项目(31572599,41376134)
【分类号】：S917.4

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