二溴海因对吉富罗非鱼血浆抗氧化酶基因表达的影响

发布时间：2018-08-07 18:17

【摘要】：研究不同时间点(1、2、4、8、16 d)及修复期(26 d)、不同浓度(0、0.06、0.3、1.5 mg/L)二溴海因(DBDMH)对雄性吉富罗非鱼血浆中抗氧化酶基因的基因毒性,筛选稳定的生物标志物。实时荧光定量PCR方法检测DBDMH对超氧化物歧化酶(sod)、谷胱甘肽-S-转移酶(gst)、谷胱甘肽还原酶(gr)、谷胱甘肽过氧化物酶1(gpx1)、过氧化氢酶(cat)基因表达的影响。中浓度组雄性吉富罗非鱼血浆抗氧化酶基因(除gr)表达受DBDMH胁迫诱导显著上调;各DBDMH浓度组1 d(sod,gst,gpx1),2 d(gst,gr)抗氧化基因表达呈现浓度依赖性下降,4 d sod,gpx1基因表达呈现浓度依赖性上升;8 d中浓度DBDMH处理组基因表达(gst,gr,gpx1)显著低于低浓度组;恢复试验阶段,中、高浓度DBDMH组各抗氧化酶基因表达均不能恢复到正常水平。0.3 mg/L DBDMH显著增强雄性吉富罗非鱼血浆所测抗氧化酶基因的表达(除1 d gpx1、cat,8和16 d gr),此浓度下抗氧化酶基因作为标志物较合适。
[Abstract]:To study the genotoxicity of dibromohydantoin (DBDMH) at different time points (1 ~ 2 ~ 2 ~ 4 ~ 4 ~ 8 ~ 8 ~ 16 days) and repair period (26 d),) (0. 06 ~ 0. 3 ~ 1. 5 mg/L) to antioxidant enzyme gene in plasma of male tilapia japonica, and to screen stable biomarkers. The effect of DBDMH on the expression of (gr), glutathione peroxidase (gpx1) and catalase (cat) (cat) in superoxide dismutase (SOD) (sod), glutathione -Stransferase (gst),) glutathione reductase (gst),) and catalase (cat) was detected by real-time fluorescence quantitative PCR. In middle concentration group, the expression of antioxidant enzyme gene (except gr) in plasma was significantly up-regulated by DBDMH stress. The expression of antioxidant genes in each DBDMH concentration group was decreased in a concentration-dependent manner on the 1st day (sodfen gpx1) 2 days (GSTN gpx1). In the 8th day, the gene expression in the DBDMH treatment group was significantly lower than that in the low concentration group, and in the recovery test stage, the gene expression in the DBDMH treatment group was significantly lower than that in the low concentration group, and in the recovery test stage, the expression of the gene in the DBDMH treatment group was significantly lower than that in the low concentration group. The expression of antioxidant enzyme genes in high concentration DBDMH group could not return to normal level. 0.3 mg/L DBDMH significantly enhanced the expression of antioxidant enzyme gene in plasma of male Gifeto tilapia (except for 1 d gpx1 catao 8 and 16 d gr),). It is more suitable as a marker.
【作者单位】：中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种与养殖生物学重点开放实验室农业部长江下游渔业资源环境科学观测实验站中国水产科学研究院内陆渔业生态环境和资源重点开放实验室;南京农业大学无锡渔业学院;
【基金】：现代农业产业技术体系专项(CARS-49) 国家农产品质量安全风险评估项目(GJFP201501004) 国家科技支撑计划(2015BAD13B03)
【分类号】：X171.5

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