长链非编码核糖核酸牛磺酸上调基因1在肺腺癌中的表达及生物学意义研究
发布时间：2018-08-07 21:17
【摘要】：目的探讨长链非编码核醣核酸牛磺酸上调基因1(lncRNA-TUG1)在肺腺癌组织中的表达及其对肺腺癌A549细胞生长的影响。方法选取2012年1月-2014年12月间于天津市第一中心医院胸外科行手术切除的肺腺癌患者的肿瘤组织及对应癌旁组织40例。运用实时荧光定量聚合酶链反应(qRT-PCR)技术检测lncRNA-TUG1在组织中的表达水平,分析lncRNA-TUG1表达与患者临床病例资料间的相关性;通过si RNA沉默人肺腺癌A549细胞中lncRNA-TUG1的表达,采用噻唑蓝实验检测沉默lncRNA-TUG1后A549细胞增殖变化,流式细胞仪检测细胞凋亡变化,qRT-PCR及Western blot检测P16表达变化。结果 lncRNA-TUG1在肺癌组织中表达水平高于对应癌旁组织(t=3.873,P=0.000),肺癌组织中lncRNA-TUG1高表达与肿瘤直径增大(P=0.033)及高TNM分期(P=0.045)相关;lncRNA-TUG1特异性si RNA转染A549细胞后可抑制细胞增殖(P=0.041)并上调凋亡细胞比例(t=3.206,P=0.007);qRT-PCR及Western blot结果证实,沉默lncRNA-TUG1后可促进P16 m RNA及蛋白表达水平(P=0.000)。结论 lncRNA-TUG1在肺腺癌组织中表达上调并与肿瘤恶性临床病理特征有关,lncRNA-TUG1可能通过下调抑癌基因P16的表达来促进肺腺癌生长。
[Abstract]:Objective to investigate the expression of long chain noncoding ribonucleic acid taurine up-regulated gene 1 (lncRNA-TUG1) in lung adenocarcinoma and its effect on the growth of lung adenocarcinoma cell line A549. Methods from January 2012 to December 2014, 40 patients with lung adenocarcinoma were selected from thoracic surgery department of Tianjin first Central Hospital. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA-TUG1 in human lung adenocarcinoma A549 cells, and to analyze the correlation between the expression of lncRNA-TUG1 and the clinical data of patients, and to silence the expression of lncRNA-TUG1 in human lung adenocarcinoma A549 cells by si RNA. The proliferation of A549 cells after silencing lncRNA-TUG1 was detected by thiazolyl blue assay, apoptosis was detected by flow cytometry and P16 expression was detected by Western blot. Results the expression level of lncRNA-TUG1 in lung cancer tissues was higher than that in corresponding adjacent tissues (Tn3.873P0. 000). The high expression of lncRNA-TUG1 in lung cancer tissues was associated with tumor diameter enlargement (Pn0. 033) and high TNM stage (P0. 045). The expression of lncRNA-TUG1 in A549 cells was inhibited by transfection of LNcRNA-TUG1 specific si RNA (P0. 041). The results of QRT-PCR and Western blot showed that the proportion of apoptotic cells (tr 3.206) was adjusted by QRT-PCR and Western blot. Silencing lncRNA-TUG1 could promote the expression of P16 m RNA and protein (P0. 000). Conclusion the expression of lncRNA-TUG1 in lung adenocarcinoma is up-regulated and related to the malignant clinicopathological features of lung adenocarcinoma, which may promote the growth of lung adenocarcinoma by down-regulating the expression of tumor suppressor gene P16.
【作者单位】： 天津中医药大学;天津市第一中心医院免疫科;
【基金】：天津市卫生局中医处课题基金项目(No:2015047)
【分类号】：R734.2
[Abstract]:Objective to investigate the expression of long chain noncoding ribonucleic acid taurine up-regulated gene 1 (lncRNA-TUG1) in lung adenocarcinoma and its effect on the growth of lung adenocarcinoma cell line A549. Methods from January 2012 to December 2014, 40 patients with lung adenocarcinoma were selected from thoracic surgery department of Tianjin first Central Hospital. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA-TUG1 in human lung adenocarcinoma A549 cells, and to analyze the correlation between the expression of lncRNA-TUG1 and the clinical data of patients, and to silence the expression of lncRNA-TUG1 in human lung adenocarcinoma A549 cells by si RNA. The proliferation of A549 cells after silencing lncRNA-TUG1 was detected by thiazolyl blue assay, apoptosis was detected by flow cytometry and P16 expression was detected by Western blot. Results the expression level of lncRNA-TUG1 in lung cancer tissues was higher than that in corresponding adjacent tissues (Tn3.873P0. 000). The high expression of lncRNA-TUG1 in lung cancer tissues was associated with tumor diameter enlargement (Pn0. 033) and high TNM stage (P0. 045). The expression of lncRNA-TUG1 in A549 cells was inhibited by transfection of LNcRNA-TUG1 specific si RNA (P0. 041). The results of QRT-PCR and Western blot showed that the proportion of apoptotic cells (tr 3.206) was adjusted by QRT-PCR and Western blot. Silencing lncRNA-TUG1 could promote the expression of P16 m RNA and protein (P0. 000). Conclusion the expression of lncRNA-TUG1 in lung adenocarcinoma is up-regulated and related to the malignant clinicopathological features of lung adenocarcinoma, which may promote the growth of lung adenocarcinoma by down-regulating the expression of tumor suppressor gene P16.
【作者单位】： 天津中医药大学;天津市第一中心医院免疫科;
【基金】：天津市卫生局中医处课题基金项目(No:2015047)
【分类号】：R734.2
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