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饲粮能氮水平对不同品种猪肉质性状及相关基因表达的影响

发布时间:2018-08-08 10:53
【摘要】:本试验旨在研究饲粮能氮水平对不同品种猪肉质性状以及肌纤维类型和细胞因子相关基因表达的影响。选取巴马香猪和长白猪共96头(48头/品种),随机分为4组(每组24头猪),分别为长白猪饲喂NRC饲粮组、长白猪饲喂我国地方猪标准(GB)饲粮组、巴马香猪饲喂NRC饲粮组、巴马香猪饲喂GB饲粮组。试验从5周龄开始,至出栏时结束。分别于保育期、生长期和肥育期末,每组选择8头体况相近猪只屠宰,采集肌肉样品分析。结果表明:生长阶段显著影响了试验猪背最长肌熟肉率和肌球蛋白重链(MyHC)Ⅱx、MyHCⅡb及白细胞介素-15(IL-15)mRNA表达水平(P0.05),且随生长阶段呈增加趋势;与长白猪相比,巴马香猪的背最长肌熟肉率、MyHCⅠmRNA表达水平显著升高(P0.05),而滴水损失显著降低(P0.05);与NRC饲粮相比,GB饲粮显著提高了生长期长白猪和巴马香猪的p H45 min(P0.05),显著上调了保育期和肥育期猪背最长肌MyHCⅠmRNA表达水平(P0.05);与GB饲粮相比,NRC饲粮显著上调了生长期猪背最长肌MyHCⅠmRNA表达水平(P0.05)。由此可见,品种和生长阶段均可显著影响肉质性状及相关基因的表达,饲粮能氮水平则主要通过与生长阶段产生互作效应影响肉质性状及肌纤维类型相关基因的表达。
[Abstract]:The purpose of this study was to study the effects of dietary energy and nitrogen levels on the quality traits, muscle fiber types and cytokine related gene expression in different varieties of pork. 96 Bama Xiang pigs and Landrace pigs (48 pigs per breed) were randomly divided into 4 groups (24 pigs in each group), which were fed NRC diet group, Landrace pig standard (GB) diet group and Bama Xiang pig NRC diet group, respectively. Bama Xiang pig was fed with GB diet group. The trial began at 5 weeks of age and ended at the end of the hurdle. During the conservation period, growth period and finishing period, 8 pigs with similar body condition were slaughtered in each group, and muscle samples were collected for analysis. The results showed that the rate of longissimus dorsi muscle cooked meat, the expression of myosin heavy chain (MyHC) 鈪,

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