羧基裂解酶基因对施氏假单胞菌N2代谢苯酚的影响

发布时间：2018-08-08 11:15

【摘要】：研究了羧基裂解酶基因对施氏假单胞菌N2降解苯酚的影响。结果表明,施氏假单胞菌N2以唯一碳源苯酚生长时,与野生菌相比,突变菌株的生长明显受到抑制,ubiD和ubiX等羧基裂解酶基因发挥重要作用,并且,就生长特性而言,ubiD基因起关键作用。降解反应期间,对开环产物进行研究发现,基因ubiD主导的是邻苯二酚间位开环,而基因ubiX主导的是邻苯二酚邻位开环。从质谱结果分析,野生菌和突变菌以苯酚为碳源生长时,均能通过羟化和羧化开环裂解途径将苯酚转化成相应的中间产物:顺,顺-己二烯二酸和2-HMS。其中,单突变株N2ubiX~-降解苯酚过程中生成的中间产物与野生菌相比,种类相等,但各中间产物含量明显低于野生菌,而单突变株N2ubiD~-和双突变菌株N2ubiX~-ubiD~-代谢苯酚的中间产物的含量及种类都明显低于野生菌。
[Abstract]:The effect of carboxyl lyase gene on N 2 degradation of phenol by Pseudomonas sp. The results showed that when Pseudomonas sp. N2 was grown as a sole carbon source phenol, the growth of mutant strains was significantly inhibited by carboxyl lyase genes such as ubiubiD and ubiX. The ubiD gene plays a key role in terms of growth characteristics. During the degradation reaction, the ring-opening products were studied. It was found that the gene ubiD dominated catechol ring opening, while the gene ubiX dominated catechol ring opening. The results of mass spectrometry showed that both wild and mutant bacteria could transform phenol into corresponding intermediate products: cis, maleic acid and 2-HMS through the pathway of hydroxylation and carboxylation ring-opening cracking when they were growing with phenol as carbon source. Among them, the intermediate products produced in the process of N _ 2ubiX- degradation of phenol by single mutant strain were equal to those of wild bacteria, but the content of intermediate products was obviously lower than that of wild bacteria. However, the contents and species of intermediate products of phenol metabolism of single mutant N2ubiD- and double mutant N2ubiX- ubiD- were significantly lower than those of wild strains.
【作者单位】：西安建筑科技大学环境与市政工程学院;陕西省微生物研究所;
【基金】：国家自然科学基金资助项目(31200094) 陕西省国际科技合作重点项目(2012KW-25) 榆林市2011年产学研合作项目
【分类号】：X703;X172

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