T-钙黏蛋白基因对人鼻咽癌CNE-2细胞增殖以及凋亡的影响

发布时间：2018-08-11 14:53

【摘要】：目的探讨T-钙黏蛋白(T-cadherin)因子水平对人鼻咽癌(NPC)细胞株CNE-2增殖及凋亡的影响。方法采用RT PCR检测Tcadherin基因含量,采用Western印迹方法检测T-cadherin蛋白表达水平,并构建T-cadherin过表达载体用于转染NPC细胞株,使CNE-2细胞株高表达T-cadherin,运用RT PCR和Western印迹方法进行验证。最后采用MTT法及流式细胞技术检测T-cadherin因子水平与CNE-2细胞增殖及凋亡是否具有相关性。结果 RT PCR和Western印迹结果显示NPC组织中T-cadherin基因含量和蛋白表达水平显著降低(P0.05);转染T-cadherin基因过表达质粒的CNE-2细胞增殖水平显著降低(P0.05);转染T-cadherin基因过表达质粒的CNE-2细胞凋亡水平显著升高(P0.05);对照组和正常组以上指标的差异无统计学意义(P0.05)。结论 T-cadherin基因具有抑制NPC细胞增殖和促进NPC细胞凋亡的作用,在NPC靶向治疗上有一定应用前景。
[Abstract]:Objective to investigate the effect of T- cadherin (T-cadherin) on the proliferation and apoptosis of human nasopharyngeal carcinoma (NPC) (NPC) cell line CNE-2. Methods RT PCR was used to detect the content of Tcadherin gene, Western blot was used to detect the expression of T-cadherin protein, and T-cadherin overexpression vector was constructed to transfect NPC cell line. T-cadherin was expressed in CNE-2 cell line. RT PCR and Western blotting methods were used to verify the expression of T-cadherin. Finally, MTT assay and flow cytometry were used to detect the correlation between the level of T-cadherin factor and the proliferation and apoptosis of CNE-2 cells. Results RT PCR and Western blotting showed that the T-cadherin gene content and protein expression level in NPC tissues were significantly decreased (P0.05), the proliferation level of CNE-2 cells transfected with T-cadherin gene overexpression plasmid was significantly decreased (P0.05), and CNE-2 fine cells transfected with T-cadherin gene overexpression plasmid were significantly decreased (P0.05). The level of apoptosis was significantly increased (P0.05), and there was no significant difference between the control group and the normal group (P0.05). Conclusion T-cadherin gene can inhibit the proliferation of NPC cells and promote the apoptosis of NPC cells, and it may be used in the targeted therapy of NPC.
【作者单位】：广东医学院附属医院肿瘤中心;
【基金】：湛江市科技攻关计划项目(No.2015B01024)
【分类号】：R739.63

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