烟草NtCBL1基因的克

发布时间：2018-08-11 14:28

【摘要】：CBL是一类Ca~(2+)感受蛋白,在植物适应或抵制逆境胁迫的过程中发挥重要的作用。从烟草品种K326中克隆到了一个CBL1的同源基因,该序列包含了一个642 bp的开放阅读框,编码一个由213个氨基酸残基组成的蛋白,预测分子量为24.5 kDa,等电点为5.03。同源性分析结果显示,该基因与林烟草CBL1、甜辣椒CBL1等具有较高的同源性,故命名为NtCBL1。生物信息学分析表明,NtCBL1具有CBL家族保守的EF-hand钙结合结构域。组织表达分析发现该基因在成熟期的根、茎、叶、花中均有表达,在根中的表达量最高。逆境胁迫实验表明,该基因表达受低钾、高盐、干旱、ABA和低温诱导调控,参与烟草生物与非生物逆境胁迫的响应。并成功构建了NtCBL1-pBI121过表达载体。研究结果为解析NtCBL1在响应逆境胁迫的功能奠定一定理论基础。
[Abstract]:CBL is a kind of Ca2 receptor protein, which plays an important role in plant adaptation or resistance to stress. A homologous gene of CBL1 was cloned from tobacco variety K326, which contains an open reading frame of 642bp and encodes a protein composed of 213 amino acid residues with a predicted molecular weight of 24.5 kDa and a isoelectric point of 5.03. The result of homology analysis showed that the gene had high homology with forest tobacco CBL1 and sweet pepper CBL1, so it was named NtCBL1. Bioinformatics analysis showed that NtCBL1 had the conserved EF-hand calcium binding domain of CBL family. Tissue expression analysis showed that the gene was expressed in the roots, stems, leaves and flowers of mature stage, and the highest expression was found in the roots. The results of stress showed that the gene expression was regulated by low potassium, high salt, drought abscisic acid (ABA) and low temperature induction, and was involved in the response of tobacco to abiotic and biological stresses. NtCBL1-pBI121 overexpression vector was successfully constructed. The results provide a theoretical basis for the analysis of the function of NtCBL1 in response to stress.
【作者单位】：四川农业大学农学院;
【基金】：植物生理学与生物化学国家重点实验室开放课题(SKLPPBKF1505,SKLPPBKF1506)~~
【分类号】：Q943.2;S572

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