河南省麦田猪殃殃对苯磺隆的抗性及ALS基因突变研究

发布时间：2018-08-13 17:18

【摘要】：为明确河南省部分地区麦田猪殃殃对苯磺隆的抗性水平及抗性靶标分子机制,对18个猪殃殃种群进行了乙酰乳酸合成酶(acetolactate synthase,ALS)离体活性测定和ALS基因突变分析。结果表明,种群PDS-1、ZK-1、LH-1对苯磺隆产生了较高的抗性,ALS离体活性测定所得I_(50)分别为11.27、10.26、8.19μmol·L~(-1),抗性指数分别为93.92、85.50、68.25。种群ZK-1的6个检测株中,ALS基因第590位的C突变为T,ALS酶第197位脯氨酸(CCC)突变为亮氨酸(CTC);种群PDS-1的6个检测株中,ALS基因第1 128位的T突变为A,ALS酶第376位天冬氨酸(GAT)全部突变为谷氨酸(GAA);种群LH-1的6个检测株中,3株未检测到突变,另外3株的ALS基因第1 128位的T突变为G,ALS酶第376位天冬氨酸(GAT)突变为谷氨酸(GAG)。靶标ALS基因突变可能是猪殃殃对苯磺隆产生高抗性的重要原因之一。
[Abstract]:In order to clarify the resistance level and target molecular mechanism of Herba plaginaris in Henan Province, the activity of acetolactate synthase (ALS) in vitro and ALS gene mutation were analyzed in 18 populations of S. instars. The results showed that PDS-1, ZK-1 and LH-1 had higher resistance to benzene, and ALS. In vitro, I_ (50) was 11.27, 10.26, 8.19 micromol L (- 1) and resistance index was 93.92, 85.50, 68.25, respectively. Among the 6 strains tested for ZK-1, C mutation at position 590 of ALS gene was T, proline mutation at position 197 of ALS enzyme was Leucine (CTC), T mutation at position 1128 of ALS gene was A, and ALS enzyme mutation at position 1128 of ALS gene was ALS enzyme. All 376 aspartic acid (GAT) mutated to glutamate (GAA); 3 of the 6 tested strains of LH-1 did not detect mutations, and 3 of the 1128th ALS mutations of G gene were G, ALS enzyme 376th aspartate (GAT) mutated to glutamate (GAG). The target ALS gene mutation may be one of the important reasons for the high resistance of CL.
【作者单位】：河南省农业科学院植物保护研究所/河南省农作物病虫害防治重点实验室/农业部华北南部作物有害生物综合治理重点实验室/河南省作物保护国际联合实验室/河南省生物农药工程研究中心;郸城县农业科学研究所;河南省农药检定站;
【基金】：河南省农业科学院自主创新专项(2013ZZ22,2016ZC43)
【分类号】：S451.221

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