TGFβ1信号通路基因多态性与宫颈癌患者放射性直肠损伤相关性的研究

发布时间：2018-08-15 13:06

【摘要】：目的:探讨TGFβ1信号通路基因TGFβ1、SMAD3及SMAD4的单核苷酸多态性与宫颈癌患者急性和慢性放射性直肠损伤的相关性及其临床意义。方法:采用回顾性研究的方法,收集了121例接受过宫颈癌放射治疗的患者,并随访他们发生放射性直肠损伤的情况。急性放射性直肠损伤按RTOG分级标准,慢性放射性肠损伤按RTOG/EORTC分级标准。应用第一代测序技术——双脱氧链终止法检测患者TGFβ1、SMAD3及SMAD4基因的单核苷酸多态性。采用SPSS18.0统计软件,以单因素及多因素二元Logistic回归分析的方法,统计TGFβ1(rs11466313,rs1800468,rs1800469,rs1800470,rs1800471)、SMAD3(rs12901499)及SMAD4(rs12456284)共7个位点的单核苷酸多态性与急性及慢性放射性直肠损伤的相关性。结果:SMAD4基因rs12456284位点多态性与慢性放射性直肠损伤的发生有显著相关性。在多因素logistic回归分析中,与AA基因型患者相比,AG基因型及GG基因型发生慢性肠炎的风险明显降低(OR=0.308,p=0.012及OR=0.121,p=0.007),但是与急性放射性直肠损伤无显著相关性(p≥0.05)。而TGFβ1(rs11466313,rs1800468,rs1800469,rs1800470,rs1800471),SMAD3(rs12901499)的多态性与急性及慢性放射性直肠损伤均无明显相关性(p≥0.05)。此外,放疗总剂量≥85Gy也可显著增加慢性放射性直肠损伤的发生风险(OR=2.406,p=0.036)。结论:SMAD4基因rs12456284位点多态性与宫颈癌患者放疗后慢性直肠损伤的发生相关,其AG基因型及GG基因型可能是慢性放射性直肠损伤的保护性因素。放疗总剂量≥85Gy是增加慢性放射性直肠损伤发生风险的一个临床因素。
[Abstract]:Objective: to investigate the association of single nucleotide polymorphisms (SNP) of TGF 尾 1 signaling gene TGF 尾 1 SMAD3 and SMAD4 with acute and chronic radiation-induced rectal injury in patients with cervical cancer and its clinical significance. Methods: 121 patients who received radiotherapy for cervical cancer were collected by retrospective study and followed up. Acute radiation-induced rectal injury was classified according to RTOG criteria and chronic radiation-induced intestinal injury was graded according to RTOG/EORTC criteria. The single nucleotide polymorphisms of TGF 尾 1 SMAD3 and SMAD4 gene were detected by the first generation sequencing technique. The single nucleotide polymorphisms (SMAD3 (rs12901499) and SMAD4 (rs12456284) of 7 loci of TGF 尾 1 (rs11466313, rs1800468, rs1800446 9, rs1800470) were analyzed by SPSS18.0 statistical software. The single nucleotide polymorphisms of SMAD3 (rs12901499) and SMAD4 (rs12456284) were correlated with acute and chronic radiation-induced rectal injury. Results the rs12456284 polymorphism of the SMAD4 gene was significantly associated with the occurrence of chronic radiation-induced rectal injury. In multivariate logistic regression analysis, the risk of chronic enteritis in patients with AG and GG genotype was significantly lower than that in patients with AA genotype (ORO 0.308, P 0.012 and ORX 0.121, P 0.007), but not significantly correlated with acute rectal radiation injury (p 鈮，

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