贵州地方黄牛不同组织中PPARγ基因表达水平研究

发布时间：2018-08-15 16:32

【摘要】：试验旨在研究威宁牛、思南牛、关岭牛和黎平牛不同组织中过氧化物酶体增殖物激活受体γ(peroxisome proliferators-activated receptorsγ,PPARγ)基因mRNA的表达差异。以这4个贵州地方品种黄牛为试验动物,提取心脏、肝脏、脾脏、肺脏、肾脏、脂肪和背最长肌组织的总RNA,设计PPARγ基因的实时荧光定量引物,以牛GAPDH基因为内参,应用实时荧光定量PCR技术检测PPARγ基因在4个品种不同组织中mRNA的相对表达量。结果显示,PPARγ基因在4个品种黄牛的心脏、肝脏、脾脏、肺脏、肾脏、脂肪和背最长肌组织中均有表达,但在脂肪组织中的表达量高于其他组织,且差异极显著(P0.01),在脾脏和肺脏中也有较高表达,而在肾脏、肝脏、心脏和背最长肌中表达量较低;不同品种PPARγ基因的表达在部分组织中存在差异。研究表明,PPARγ基因在不同组织中的表达量存在差异,可能与其在不同组织中的功能有关,而品种对于PPARγ基因的表达影响不大。
[Abstract]:The purpose of this study was to study the expression of peroxisome proliferator activated receptor 纬 (peroxisome proliferators-activated receptors 纬) mRNA in different tissues of Weining cattle, Sinan cattle, Guanling cattle and Liping cattle. The total RNAs of heart, liver, spleen, lung, kidney, fat and longissimus dorsi muscle tissue were extracted from the four Guizhou native cattle as experimental animals. The real-time fluorescent quantitative primers of PPAR 纬 gene were designed, and the bovine GAPDH gene was used as internal reference. The relative expression of PPAR 纬 gene in different tissues of four cultivars was detected by real-time fluorescence quantitative PCR. The results showed that PPAR 纬 gene was expressed in heart, liver, spleen, lung, kidney, fat and longissimus dorsi muscle of four cattle, but the expression level in adipose tissue was higher than that in other tissues. The difference was significant (P0.01), and the expression of PPAR 纬 gene was higher in spleen and lung, but lower in kidney, liver, heart and longissimus dorsi muscle, and the expression of PPAR 纬 gene was different in some tissues. The results showed that there were differences in the expression of PPAR 纬 gene in different tissues, which might be related to its function in different tissues, but the variety had little effect on the expression of PPAR 纬 gene.
【作者单位】：高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州省动物遗传育种与繁殖重点实验室;贵州大学动物科学学院;贵州大学生命科学学院;
【基金】：国家自然科学基金面上项目(31571279) 贵州省科技厅农业攻关项目(黔科合NY字[2012]3008号)
【分类号】：S823.81

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