水稻抗稻瘟病基因Pita特异性分子标记开发及应用
发布时间：2018-08-16 16:54
【摘要】：Pita是一个对稻瘟病生理小种具有广谱抗性的基因,通过对Pita抗病等位基因与感病等位基因编码区进行PCR扩增、测序与序列比对,在编码区的+2 001 bp即内含子中鉴定到一处特异性编码序列(抗、感病等位基因分别为GCC和CTAT),针对该目标位点,本研究利用两对交叉引物PCR(PCR with confronting two-pair primers,PCR-CTPP)方法开发出一套鉴定该目标位点的共显性标记用于鉴定Pita等位基因类型,结果表明该标记特异性强、稳定、结果准确、耗时少、成本低廉,可广泛应用于分子标记辅助选择育种或水稻资源中对Pita基因型鉴定、筛选的应用。
[Abstract]:Pita is a broad-spectrum resistance gene to rice blast physiological races. The coding region of Pita resistance allele and susceptible allele was amplified by PCR, sequenced and sequenced. A specific coding sequence was identified in the 2 001 BP intron of the coding region. The anti- and susceptible alleles were GCC and CTAT), for the target site, respectively. In this study, a set of co-dominant markers to identify the target locus was developed by using two pairs of cross-primer PCR (PCR with confronting two-pair primer PCR-CTPP to identify the allele type of Pita. The results showed that the marker was specific, stable, accurate, time-consuming and low cost. It can be widely used in molecular marker-assisted selection breeding or in the identification and screening of Pita genotypes in rice resources.
【作者单位】： 湖北省农业科学院粮食作物研究所粮食作物种质创新与遗传改良湖北省重点实验室;
【基金】：“七大农作物育种”试点专项(2016YFD0102103) 科技部国际合作专项(2011DFB31620) 国家973计划课题(2013CBA01405) 湖北省农业科学院青年科学基金项目(2015NKYJJ02)共同资助
【分类号】：S435.111.41
本文编号：2186597
[Abstract]:Pita is a broad-spectrum resistance gene to rice blast physiological races. The coding region of Pita resistance allele and susceptible allele was amplified by PCR, sequenced and sequenced. A specific coding sequence was identified in the 2 001 BP intron of the coding region. The anti- and susceptible alleles were GCC and CTAT), for the target site, respectively. In this study, a set of co-dominant markers to identify the target locus was developed by using two pairs of cross-primer PCR (PCR with confronting two-pair primer PCR-CTPP to identify the allele type of Pita. The results showed that the marker was specific, stable, accurate, time-consuming and low cost. It can be widely used in molecular marker-assisted selection breeding or in the identification and screening of Pita genotypes in rice resources.
【作者单位】： 湖北省农业科学院粮食作物研究所粮食作物种质创新与遗传改良湖北省重点实验室;
【基金】：“七大农作物育种”试点专项(2016YFD0102103) 科技部国际合作专项(2011DFB31620) 国家973计划课题(2013CBA01405) 湖北省农业科学院青年科学基金项目(2015NKYJJ02)共同资助
【分类号】：S435.111.41
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1 李时飞;四川水稻品种中抗稻瘟病基因Pita多样性[D];四川农业大学;2012年
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