大豆品种郑97196对疫霉根腐病的抗性遗传分析及基因定位

发布时间：2018-08-21 07:16

【摘要】：由大豆疫霉引起的大豆疫霉根腐病是一种世界性大豆病害,对产量品质影响极大。利用抗病品种进行防治是目前最经济、有效的方法。大豆对疫霉菌株的抗性分为由单基因控制的完全抗性和由多基因控制的部分抗性两种。以对多个大豆疫霉生理小种具有抗性的大豆品种郑97196作为抗性亲本与大豆感病品系X242003杂交构建F_(2∶3)重组自交家系群体,用大豆疫霉菌株He N35对亲本及F_(2∶3)群体进行抗性遗传分析并利用SSR技术对郑97196的抗性基因进行定位。结果表明:郑97196对大豆疫霉抗性是由一对显性单基因控制的,抗病对感病表现为显性,再用9种毒力公式不同的疫霉菌株分别接种郑97196和14个国内外公认的含有单个抗病基因的大豆品种(鉴别寄主),观察并记录抗性反应类型,结果显示郑97196的抗性反应类型与14个鉴别寄主有所不同,推测其可能含有新的抗病基因,暂命名为Rps Zheng。通过SSR分子作图分析,该基因位于大豆分子遗传图谱N连锁群上satt485和satt584之间,与这两个标记之间的距离分别为2.1和3.7cM。
[Abstract]:Phytophthora sojae root rot caused by Phytophthora sojae is a worldwide disease of soybean, which has a great impact on Yield and quality. It is the most economical and effective method to control the disease-resistant varieties. Resistance of soybean to Phytophthora sojae can be divided into complete resistance controlled by single gene and partial resistance controlled by multiple genes. Zheng 97196, a soybean variety with resistance to Phytophthora sojae, was crossed with soybean susceptible strain X242003 to construct F_ (2:3) recombinant inbred family population. The resistance genetic analysis of the parents and F_ (2:3) population of Phytophthora sojae strain He N35 was carried out and the resistance gene of Zheng 97196 was mapped by SSR technique. Resistance to Phytophthora sojae was controlled by a pair of dominant single genes. Resistance to Phytophthora sojae was dominant. Nine strains of Phytophthora sojae with different virulence formulas were inoculated with Zheng 97196 and 14 soybean cultivars (identification hosts) with a single resistance gene recognized at home and abroad. The resistance types were observed and recorded. The results showed that Zheng 97196 was resistant to Phytophthora so The reaction type was different from that of the 14 identified hosts. It was speculated that the gene might contain a new resistance gene, tentatively named Rps Zheng. SSR molecular mapping analysis showed that the gene was located between satt485 and satt584 in the N linkage group of soybean molecular genetic map, and the distances between the two markers were 2.1 and 3.7 cM, respectively.
【作者单位】：南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室;
【基金】：国家公益性行业(农业)科研专项经费(201303018) 优质高产高油大豆种质创新与新品种选育(BE2013350) 江苏省自然科学基金项目(BK2011637和BK20130684) 国家现代农业产业技术体系(CARS-04-PS10) 江苏省现代作物生产协同创新中心
【分类号】：S435.651

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