拟南芥锌指结构域ABRv1基因的功能研究
发布时间：2018-08-21 08:47
【摘要】：为了分析拟南芥脱落酸响应蛋白RING-V1基因(ABRv1)的功能,构建了高表达载体pBI121-ABRv1,经农杆菌转化,花序浸染后得到T0代转基因种子,并经卡那霉素板筛选得到转基因苗,再经过两代的筛选获取了纯合的高表达株系ABRv1.通过DNA及RNA水平鉴定了ABRv1基因的T-DNA插入突变体abrv1.对突变体、高表达转基因株系及野生型进行ABA诱导处理,突变体的萌发率不足10%,野生型萌发率为40%,而过表达株系萌发率为67%;突变体株系气孔几乎全部关闭,过表达株系气孔关闭不明显,大小是突变体株系的2~3倍.结果表明ABRv1基因在拟南芥的ABA信号应答响应中可能起负调控作用.
[Abstract]:In order to analyze the function of abscisic acid responsive protein RING-V1 gene (ABRv1) in Arabidopsis thaliana, a high expression vector pBI121-ABRv1 was constructed. Transgenic seeds of T 0 generation were obtained by Agrobacterium tumefaciens transformation, inflorescence soaking, and transgenic seedlings were screened by kanamycin plate. After two generations of screening, the homozygous high expression line ABRv1 was obtained. The T-DNA insertion mutant abrv1 of ABRv1 gene was identified by DNA and RNA levels. The germinating rate of mutant, high expression transgenic line and wild type was less than 10%, the germination rate of wild type was 40 and that of over-expressed line was 67%, and the stomata of mutant were almost all closed. The stomatal closure of the overexpressed lines was not obvious, and the size of the overexpressed lines was 2 ~ 3 times as large as that of the mutant lines. The results suggest that ABRv1 gene may play a negative role in the response to ABA signaling in Arabidopsis thaliana.
【作者单位】： 四川大学生命科学学院生物资源与生态环境教育部重点实验室;
【基金】：国家自然科学基金(31171586) 973计划(2015CB755702)
【分类号】：Q943.2
，
本文编号：2195187
[Abstract]:In order to analyze the function of abscisic acid responsive protein RING-V1 gene (ABRv1) in Arabidopsis thaliana, a high expression vector pBI121-ABRv1 was constructed. Transgenic seeds of T 0 generation were obtained by Agrobacterium tumefaciens transformation, inflorescence soaking, and transgenic seedlings were screened by kanamycin plate. After two generations of screening, the homozygous high expression line ABRv1 was obtained. The T-DNA insertion mutant abrv1 of ABRv1 gene was identified by DNA and RNA levels. The germinating rate of mutant, high expression transgenic line and wild type was less than 10%, the germination rate of wild type was 40 and that of over-expressed line was 67%, and the stomata of mutant were almost all closed. The stomatal closure of the overexpressed lines was not obvious, and the size of the overexpressed lines was 2 ~ 3 times as large as that of the mutant lines. The results suggest that ABRv1 gene may play a negative role in the response to ABA signaling in Arabidopsis thaliana.
【作者单位】： 四川大学生命科学学院生物资源与生态环境教育部重点实验室;
【基金】：国家自然科学基金(31171586) 973计划(2015CB755702)
【分类号】：Q943.2
，
本文编号：2195187
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