麻疯树基因JcKNOX1的克隆和表达调控分析

发布时间：2018-08-21 08:31

【摘要】：KNOX同源异型盒基因在植物生长发育中扮演着极其重要的角色,首次从大戟科植物麻疯树cDNA中克隆到一个同源异型盒基因,命名为JcKNOX1.其开放阅读框全长693bp,编码230个氨基酸残基,预测蛋白质等电点pI=6.13,分子量PA=25.92kD.聚类分析表明,JcKNOX1与毛白杨在进化上具有最近的亲缘关系,其次是甜橙.荧光定量PCR检测发现,JcKNOX1基因在麻疯树植株各个器官中均有表达,幼嫩部位表达量明显高于成熟器官,表达量依次为:茎尖嫩叶茎叶花叶柄根;对麻疯树幼苗进行ABA和低温胁迫发现,JcKNOX基因表达量明显增加.上游调控元件预测分析表明,该基因可能在麻疯树光调节、激素调控、花粉基因特异性表达、逆境调控等重要生物学过程中具有重要作用.
[Abstract]:KNOX homotypic box gene plays an important role in plant growth and development. A homologous box gene named JcKNOX1 was cloned from cDNA of Euphorbiaceae plant Jatropha curcas for the first time. Its open reading frame is 693 BP, encoding 230 amino acid residues, predicting protein isoelectric point Pi 6.13, and molecular weight PAN 25.92 KD. Cluster analysis showed that JcKNOX1 had the closest phylogenetic relationship with Populus tomentosa, followed by sweet orange. Fluorescence quantitative PCR analysis showed that JcKNOX1 gene was expressed in all organs of Jatropha curcas. The expression of JcKNOX1 in young parts was significantly higher than that in mature organs, and the order of expression was as follows: stem, leaf, stem and petiole root of stem tip, young leaf, stem and leaf; The expression of JcKNOX gene in Jatropha curcas seedlings was significantly increased under ABA and low temperature stress. The prediction analysis of upstream regulatory elements indicates that the gene may play an important role in light regulation, hormone regulation, pollen gene specific expression, stress regulation and other important biological processes in Jatropha curcas.
【作者单位】：四川大学生命科学学院生物资源与生态环境教育部重点实验室;西昌学院动物科学学院;
【基金】：国家“十二五”重大科技支撑计划课题(2011BAD22B08) 四川省教育厅项目(08ZB015)
【分类号】：Q943.2

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