嗜酸乳杆菌zrx02的plnD基因克隆及生物信息学分析

发布时间：2018-08-22 12:00

【摘要】：目的:对zrx02嗜酸乳杆菌细菌素编码基因进行克隆与序列分析。方法:对分离出的菌株进行16S rRNA鉴定,并根据已报道的plnD编码产物基因设计引物,以该菌基因组DNA为模板进行PCR扩增和测序,利用Pro Param tool,TMHMM等在线软件对编码蛋白进行生物信息学分析。结果:该菌为嗜酸乳杆菌,命名为嗜酸乳杆菌zrx02,该菌含有plnD基因,通过与Gen Bank中相关的抗菌素基因进行比对,发现其基因序列与Gen Bank库中的Lactobacillus plantarum strain(WP 016526896.1)plnD gene的同源性达到100%。生物信息学分析显示该基因全长355 bp,编码92个氨基酸,相对分子量10.54 ku,理论等电点9.26,该基因编码蛋白是稳定蛋白,不具有明显的跨膜结构,二级结构主要有α-螺旋、无规则卷曲和延伸链组成,三级结构结果显示plnD蛋白模型与Streptococcus pneumoniae中的ComE同源性最高。结论:对嗜酸乳杆菌zrx02 plnD基因的克隆与分析,为进一步探究细菌素的形成机制奠定理论基础。
[Abstract]:Objective: to clone and sequence the bacteriocin encoding gene of Lactobacillus acidophilus zrx02. Methods: the isolated strain was identified by 16s rRNA, and primers were designed according to the reported plnD coding product gene. The genomic DNA was used as template for PCR amplification and sequencing. Pro Param tool-TMHMM and other online software were used for bioinformatics analysis of the encoded protein. Results: Lactobacillus acidophilus was named as Lactobacillus acidophilus zrx02. It contained plnD gene. By comparing with the antimicrobial gene related to Gen Bank, it was found that the homology of the gene sequence with Lactobacillus plantarum strain (WP 016526896.1) plnD gene in Gen Bank library was 100%. Bioinformatics analysis showed that the gene was 355 BP in length, encoding 92 amino acids, the relative molecular weight was 10.54 ku. the theoretical isoelectric point was 9.26. The protein encoded by the gene was stable and had no obvious transmembrane structure, and the secondary structure was mainly 伪 -helix. The results of three-level structure showed that the plnD protein model had the highest homology with ComE in Streptococcus pneumoniae. Conclusion: the cloning and analysis of zrx02 plnD gene of Lactobacillus acidophilus lay a theoretical foundation for further exploring the formation mechanism of bacteriocin.
【作者单位】：河南科技学院食品学院;云南商务信息工程学校;
【基金】：河南省高校重点科研项目(18A550006) 河南省高校科技创新人才支持计划(17HASTIT037) 河南科技学院高层次人才科研项目(2015016)
【分类号】：Q78;TS201.3

【相似文献】