729例贵阳市育龄妇女弓形虫感染状况调查和基因分型

发布时间：2018-08-22 13:24

【摘要】：目的:了解贵州省729例育龄妇女弓形虫感染状况,并鉴定弓形虫基因型,为贵州省育龄妇女弓形虫感染的防治提供依据。方法:采用酶联免疫吸附试验(ELISA法)检测729例育龄妇女外周血标本弓形虫循环抗原(CAg)、抗弓形虫抗体Ig G、Ig M。利用聚合酶链反应(PCR技术)扩增弓形虫特异性基因片段(529 bp高重复序列)进行核酸检测,阳性标本则用多重巢式—聚合酶链式反应-限制性片段长度多态性分析(Mn-PCR-RFLP)方法对弓形虫进行基因分型。结果:479例孕妇外周血血浆中,CAg、抗弓形虫抗体Ig G和Ig M阳性率分别为0.63%,7.1%,0.84%。其中正常妊娠样本阳性率分别为0.56%,4.47%,0.56%,有异常妊娠史样本阳性率分别为0.83%,14.87%,1.65%。250例健康对照样本抗弓形虫抗体Ig G阳性率为4%,未发现弓形虫CAg及抗Ig M抗体。对比血清学总阳性率,有异常妊娠史组与正常妊娠组差异有统计学意义(χ2=16.003,P0.05),有异常妊娠史组与健康对照组差异有统计学意义(χ2=18.992,P0.05),正常妊娠史组与健康对照组差异无统计学意义(χ2=0.790,P0.05)。在所有检测样本中发现正常妊娠组1例,异常妊娠史组2例样本的弓形虫核酸检测呈阳性,健康对照组中未检测到弓形虫核酸。对以上3例核酸阳性标本进行Mn-PCRRFLP弓形虫基因分型,经鉴定为非典型虫株,即Chinese 1型(Toxo DB#9)。结论:育龄妇女弓形虫感染以慢性或隐性感染为主,近期感染或急性感染者少见。异常妊娠与弓形虫感染密切相关,Chinese 1型弓形虫虫株可能是贵州育龄妇女人群中感染流行的优势虫株。临床产前诊断时期应加强对弓形虫感染的防治。
[Abstract]:Objective: to investigate the infection status of Toxoplasma gondii in 729 women of childbearing age in Guizhou Province and to identify the genotype of Toxoplasma gondii so as to provide evidence for the prevention and treatment of Toxoplasma gondii infection among women of childbearing age in Guizhou Province. Methods: enzyme linked immunosorbent assay (ELISA) was used to detect the circulating antigen of Toxoplasma gondii (CAg),) against Toxoplasma gondii in 729 women of childbearing age. The specific gene fragment of Toxoplasma gondii (529 BP high repeat sequence) was amplified by polymerase chain reaction (PCR) for nucleic acid detection. Toxoplasma gondii was genotyped by multiplex nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP). Results the positive rates of CAG and IgM in peripheral blood plasma of 479 pregnant women were 0.63 and 0.84, respectively. The positive rates of normal pregnancy samples were 0.56 and 4.470.56.The positive rates of abnormal pregnancy samples were 0.8314.870.650.250 healthy controls. The positive rates of anti-Toxoplasma gondii antibody (IgG) were not found in the samples of Toxoplasma gondii CAg and anti-IgM. Compared with the total positive rate of serology, there was significant difference between the normal pregnancy group and the abnormal pregnancy history group (蠂 ~ (2 +) 16.003), the difference between the abnormal pregnancy history group and the healthy control group (蠂 ~ (2) was statistically significant (蠂 ~ (2), but there was no significant difference between the normal pregnancy history group and the healthy control group (蠂 ~ (2) 0.79 0). Toxoplasma nucleic acid was detected in 1 case of normal pregnancy group and 2 cases of abnormal pregnancy history group, but no Toxoplasma nucleic acid was detected in healthy control group. Mn-PCRRFLP Toxoplasma gondii genotyping was carried out in the above 3 nucleic acid positive specimens and identified as Chinese 1 (Toxo DB#9) strain. Conclusion: Toxoplasma gondii infection is mainly chronic or recessive infection in women of childbearing age. Abnormal pregnancy is closely related to Toxoplasma gondii infection. The strain of Toxoplasma gondii type 1 may be the dominant strain of infection in women of childbearing age in Guizhou. Prevention and treatment of Toxoplasma gondii infection should be strengthened during prenatal diagnosis.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R531.8;R440

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