氮素和Wx基因缺失对小麦淀粉生物合成的影响

发布时间：2018-08-22 18:03

【摘要】：【目的】通过不同氮素施用水平,明确氮肥水平及灌浆时期对小麦Wx近等基因系籽粒淀粉含量的影响。【方法】以宁麦14为背景的8个小麦Wx近等基因系为材料,不施底肥、在三叶期追施0、150、300、450、600kg/hm~2的尿素作为氮素梯度,测定灌浆7、14、21、28、35 d的小麦籽粒直链、支链淀粉和总淀粉含量。【结果】Wx基因型、灌浆时间对小麦籽粒的直链淀粉、支链淀粉和总淀粉含量产生显著影响,而氮素水平未引起淀粉含量及成分产生显著变化;淀粉合成在花后10 d以后极大加快、而到灌浆结束又明显趋缓。【结论】3个Wx基因直链淀粉的合成能力Wx-B1Wx-D1Wx-A1,其中Wx-D1和Wx-A1基因的综合作用与Wx-B1基本相当。
[Abstract]:[objective] to determine the effect of nitrogen fertilizer level and grain filling stage on the grain starch content of wheat WX near isogenic lines through different nitrogen application levels. [methods] eight WX near-isogenic lines of wheat with Ningmai 14 as background were used as materials, and no basal fertilizer was applied. The content of amylose, amylopectin and total starch in grain of wheat with grain filling (7141421C 2835d) was determined by applying urea of 0150300450600kg/hm~2 at three-leaf stage as nitrogen gradient. [results] WX genotype and grain filling time were used to determine the amylose content of wheat grain, [results] WX genotypes and grain filling time were used to determine the amylose content of wheat grain. Amylopectin and total starch content were significantly affected, while nitrogen level did not cause significant changes in starch content and composition, starch synthesis was greatly accelerated 10 days after anthesis. By the end of filling, the synthesis ability of Wx-B1Wx-D1Wx-A1Wx-A1Wx-A1Wx-B1Wx-D1Wx-A1Wx-A1Wx-B1Wx-D1Wx-A1Wx-A1Wx-B1Wx-D1Wx-A1Wx-A1Wx-A1@@
【作者单位】：云南农业大学农学与生物技术学院;保山学院资源环境学院;临沧市农业科学研究所;曲靖市农业科学院;江苏里下河地区农业科学研究所;
【基金】：云南省科技计划项目(2015BB016) 昆明市科技计划项目(2015-1-H-00308)
【分类号】：S512.1

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