西花蓟马取食对菜豆不同部位叶片防御基因表达的影响

发布时间：2018-08-22 18:42

【摘要】：【目的】本研究旨在探究锉吸式口器害虫西花蓟马Frankliniella occidentalis取食诱导的菜豆Phaseolus vulgaris木植株系统防御反应的分子机制。【方法】利用荧光定量PCR技术,检测西花蓟马分别在稳定取食菜豆植株2 h后的24,48,72和96 h菜豆植株不同部位(上部、中部和下部)叶片中茉莉酸信号转导途径和水杨酸信号转导途径防御相关基因脂氧合酶基因(LOX)、苯丙氨酸解氨酶基因(PAL)和β-1,3-葡聚糖酶基因(PR-2)相对表达量的变化。【结果】西花蓟马取食菜豆植株后,受害叶片及上部和下部健康叶片中LOX,PAL和PR-2均被显著诱导表达,均以受害叶片防御酶基因表达量的变化较大,并且表达量在相同叶片不同时间下的变化不同。中部受害叶片中LOX的相对表达量均显著高于未接虫的对照植株,并随西花蓟马为害时间的延长逐渐升高,在96 h时为对照的209.54倍;随着时间的延长LOX表达量在上部和下部健康叶片呈现升高-降低-升高的趋势。PAL的表达量在西花蓟马为害植株的中部受害叶片和上部健康叶片均于48 h时出现最大值,分别为对照的52.70倍和41.20倍;但在下部健康叶片于72 h时达到最大值,为对照的47.06倍。PR-2表达量在受害株的上部健康叶片于48 h时出现最大值,在中部受害叶片和下部健康叶片均于96 h时达到最大值,但在24 h时在下部健康叶片中受到抑制。【结论】西花蓟马取食能显著诱导菜豆植株茉莉酸和水杨酸信号转导途径相关防御酶基因LOX,PAL和PR-2的表达,并在菜豆植株不同部位叶片产生系统抗性。
[Abstract]:[objective] to explore the molecular mechanism of systemic defense response induced by Frankliniella occidentalis, a rasping mouthpiece pest, in Phaseolus vulgaris plantlets. [methods] fluorescence quantitative PCR technique was used. The different parts (upper part) of vegetable bean plant were detected at 244872 and 96 h after stable feeding for 2 h. Changes in the relative expression levels of Jasmonic Acid signal Transduction Pathway and Salicylic Acid signal Transduction Pathway Defense related Gene Lipoxygene-associated Gene (LOX), phenylalanine Ammonia-lyase Gene (PAL) and 尾 -1 3-glucanase Gene (PR-2) in the Middle and Lower part of the leaves. [ Results: after feeding on bean plants, thrips of western flowers, The expression of LOXPAL and PR-2 in the injured leaves and the upper and lower healthy leaves were significantly induced. The expression of defense enzyme genes in the injured leaves varied greatly, and the expression levels varied at different time points in the same leaves. The relative expression of LOX in the middle damaged leaves was significantly higher than that in the control plants without inoculation, and increased gradually with the prolongation of the damage time of the western flower thrips, and was 209.54 times of that of the control at 96 h. With the extension of time, the expression of LOX increased, decreased and increased in the upper and lower healthy leaves. The expression of pal reached the maximum at 48 h in the middle injured leaves and the upper healthy leaves of the plants damaged by thrips sibirica. The expression of PR-2 was 52.70 times and 41.20 times of the control, respectively, but reached the maximum at 72 h in the lower healthy leaves, and the maximum expression of PR-2 was 47.06 times at 48 h in the upper healthy leaves of the affected plants. The maximum value of damaged leaves and lower healthy leaves was reached at 96 h. But it was inhibited in the lower part of the leaves at 24 h. [conclusion] the feeding of thrips can significantly induce the expression of LOXPAL and PR-2 genes related to the signal transduction pathway of jasmonic acid and salicylic acid in bean plants. Phylogenetic resistance was produced in different parts of soybean leaves.
【作者单位】：贵州大学昆虫研究所贵州省山地农业病虫害重点实验室;
【基金】：国家自然科学基金项目(31160367) 贵州省农业攻关项目(黔科合NY[2015]3014-1号)
【分类号】：S436.43

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