羊IL-2基因和羊口疮病毒B2L基因真核表达重组质粒联合免疫小鼠的效果评价

发布时间：2018-08-24 18:17

【摘要】：为评价羊白介素2(IL-2)基因和羊口疮病毒(OrfV)B2L基因真核表达重组质粒联合接种小鼠的免疫效果,本研究将pVAX1-B2L、pVAX1-B2L+pVAX1-IL-2及空载体pVAX1和PBS对照组通过肌肉注射的方式免疫KM小鼠,并采用ELISA方法和MTT方法检测免疫小鼠的体液和细胞免疫反应。结果表明,pVAX1-B2L+pVAX1-IL-2联合免疫组小鼠血清抗体效价和IL-2水平均显著高于pVAX1-B2L免疫组,与pVAX1和PBS对照组相比差异极显著(p0.01)。而且pVAX1-B2L+pVAX1-IL-2免疫组的脾淋巴细胞增殖水平高于pVAX1-B2L免疫组、空载体pVAX1组和PBS对照组(p0.01)。因此,pVAX1-B2L+pVAX1-IL-2联合免疫KM小鼠可以诱导特异性体液免疫和细胞免疫,并且IL-2具有免疫佐剂的作用,为进一步将其用于Orf的防制奠定了基础。
[Abstract]:In order to evaluate the immune effect of recombinant eukaryotic expression plasmid of sheep interleukin 2 (IL-2) gene and sheep mouth sore virus (OrfV) B 2L) gene in mice, pVAX1-B2L,pVAX1-B2L pVAX1-IL-2 and empty vector pVAX1 and PBS control group were injected intramuscularly into KM mice. The humoral and cellular immune responses of immunized mice were detected by ELISA and MTT methods. The results showed that the serum antibody titers and IL-2 levels of mice immunized with pVAX1-B2L pVAX1-IL-2 were significantly higher than those of pVAX1-B2L immunized mice, and the differences were significant compared with those of pVAX1 and PBS control groups (p0.01). The proliferation level of splenic lymphocytes in pVAX1-B2L pVAX1-IL-2 immunized group was higher than that in pVAX1-B2L immunized group, empty vector pVAX1 group and PBS control group (p0.01). Therefore, combined immunization with pVAX1-B2L pVAX1-IL-2 can induce specific humoral immunity and cellular immunity in KM mice, and IL-2 has the function of immune adjuvant, which lays a foundation for further application of pVAX1-B2L pVAX1-IL-2 in the prevention and control of Orf.
【作者单位】：贵州大学动物科学学院;贵州省动物疫病研究所;重庆市畜牧科学院;
【基金】：贵州省科学技术基金项目[黔科合LH字(2014)7667] 贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队[黔科合人才团队(2015)4016号]
【分类号】：S852.65

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