Mmu-143调控牙鲆Tyrp1a基因表达及牙鲆白化皮肤细胞系的建立
[Abstract]:Paralichthys olivaceus is an important mariculture fish in the north of China. In order to study the molecular regulation mechanism of albinism in Paralichthys olivaceus, the albino and primary color samples of Paralichthys olivaceus were used as materials, and the transcriptome and microRNA (miRNA) depth sequencing data were obtained. To study the mechanism of albinism in Paralichthys olivaceus at the transcriptional and post-transcriptional levels. By combined analysis of mRNA and miRNA, the key miRNA mmu-143 and its target gene Tyrp1. of albino Paralichthys olivaceus were screened and identified. The regulation mechanism of miRNA mmu-143 during albinism was studied by verifying and analyzing the expression pattern of albino target gene and miRNA mmu-143 in Paralichthys olivaceus, and the ratio of albinism of flounder Paralichthys olivaceus was increased after treated with thyroid hormone T3. The quantitative results of up-regulated Complement component C8beta chain (c8b) complement component C9 (c9) Myoblast determination protein1 homolog (myod) and down-regulated ATP-dependent 6-phosphofructokinase (pfkm) ATP-dependent 6-phosphofructokinase (pfkm) Melanotransferrin (mfi2) Somatotropin (gh) were consistent with those of high throughput sequencing, and the primary color and albino skin of Paralichthys olivaceus were used as materials. Skin cell lines were established and named JFSK_wt and JFSK_alb, and cell growth curve, karyotype analysis, virus sensitivity, transfection and albinism related gene expression were studied. The results showed that the two cell lines could provide an effective cell platform for the functional study of target gene or microRNA. The results of this paper provide important basic data for revealing the molecular mechanism of albinism in Paralichthys olivaceus (Paralichthys olivaceus).
【学位授予单位】:上海海洋大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:S917.4
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